通过室内静态模拟实验,研究了在不同浓度的腐植酸(Humic acid,HA)条件下,四溴双酚A(tetrabromobisphenol A,TBBPA)在金鱼藻中的生物富集及对金鱼藻氧化胁迫的影响。结果表明,HA的存在降低了TBBPA在金鱼藻中的富集;低浓度HA及0.5mg·L^-1TBBPA共存条件下,金鱼藻组织中自由基强度、还原型谷胱甘肽(GSH)含量以及超氧化物歧化酶(SOD)活性均恢复到对照水平;加入高浓度的HA及0.5mg·L^-1TBBPA,金鱼藻组织中自由基强度显著增加,GSH含量受到抑制,SOD活性受到显著诱导;氧化型谷胱甘肽含量没有显著差异。考虑TBBPA的生物有效性和生态毒性时,不能忽视环境因子如HA的影响。
Under simulated static conditions in laboratory, the effects of humic acid (HA) on bioaccumulation of tetrabromobisphenol A (TBBPA) and oxidative stress induced by TBBPA in Coontail Ceratophyllum demersum L. were studied. Eight gram fresh weight of C. demersum were exposed to 0.5 mg.L^-1 TBBPA in glass beakers with 3 L 10% Hoagland's solution containing 0, 0.04, 0.20, 0.40, 0.80 and 2.0 mg.L^-1(DOC )HA, respectively, for 14 d under laboratory conditions. Controls were beakers containing only 10% Hoagland's solution without TBBPA and HA addition. The results showed that the presence of HA reduced the bioaccumulation of TBBPA in C. demersum, by 25.2% at 2.0 mg.L^-1 HA. In the absence of HA, 0.5 mg.L^-1 TBBPA increased signal intensity of free radicals and activity of superoxide dismutase (SOD)and decreased Glutathione( GSH ) content in C. demersum. At low concentrations of HA and TBBPA, the signal intensity of free radicals, GSH content, and SOD activity resumed to the control level. At higher concentrations of HA and TBBPA, the signal intensity of free radicals and SOD activity increased significantly, whereas the GSH content decreased significantly, as compared to the control. The oxidized glutathione(GSSG) contents were not significantly different at the various concentrations of HA.