中文摘要：

目的研究亚慢性低剂量砷暴露对大鼠的内分泌干扰作用。方法 200只SD大鼠按雄性、雌性均随机分为5组（对照组,0.05、0.1、0.2和0.4μg/m L组）,每组雌雄各20只,适应性饲养一周后,经饮水砷染毒分别于110 d和194 d后采用放射免疫法测定血清激素水平。结果不同浓度砷暴露组大鼠血清激素含量发生变化,饲养110 d各组雄鼠雄激素（T）依次为（4.80±4.26）、（4.54±3.31）、（1.27±1.51）、（1.93±2.23）和（4.83±2.31）ng/m L（P=0.05）;各剂量组雌鼠甲状腺素（T4）含量依次为：（40.20±7.10）、（40.85±8.66）、（46.49±8.52）、（44.66±11.83）和（26.80±8.12）ng/m L,其中0.4μg/m L组雌鼠T4含量明显降低（P〈0.05）;各剂量组雌鼠皮质醇（Cort）含量依次为（312.10±10.61）、（333.15±15.73）、（341.13±19.25）、（342.59±25.96）和（314.84±16.76）ng/m L,与对照组相比,染毒组雌鼠Cort均升高,且0.05、0.1和0.2μg/m L组均显著升高（P〈0.05）。饲养194 d各种雄鼠T4水平依次为（23.06±5.54）、（33.18±5.29）、（34.23±4.15）、（37.19±4.13）和（39.92±6.27）ng/m L,与对照组比较,各染毒组雄鼠T4均显著升高（P〈0.05）;各组雌鼠T3水平依次为（0.84±0.12）、（0.89±0.14）、（0.83±0.15）、（0.96±0.16）和（0.75±0.11）ng/m L,其中0.2μg/m L组雌鼠T3显著升高（P〈0.05）。其他各染砷组大鼠激素未发生明显变化（P〉0.05）。结论亚慢性低剂量砷暴露对SD大鼠具有轻微的内分泌干扰作用。

英文摘要：

Objective To study the effect on endocrine system of SD rats exposed to low-dose arsenic. Methods A subchronic test on rats which were divided into five groups randomly（ the control group,0. 05,0. 1,0. 2,0. 4 μg / m L）,20 rats each group,half male and female. A total of 200 rats were allowed to acclimate for 1 week and their hormones level were measured by RIA method after 110 and 194 days. Results The hormones level of rats was disordered in different groups. The T level of male rats exposedto arsenic for 110 days were（ 4. 80 ± 4. 26）,（ 4. 54 ± 3. 31）,（ 1. 27 ± 1. 51）,（ 1. 93 ±2. 23） and（ 4. 83 ± 2. 31） ng / m L（ P = 0. 05）,respectively. The T4 level of each female group were（ 40. 20 ± 7. 10）,（ 40. 85 ± 8. 66）,（ 46. 49 ± 8. 52）,（ 44. 66 ± 11. 83） and（ 26. 80 ± 8. 12） ng /m L,compared with control group,0. 4 μg /m L group decreased obviously（ P〈0. 05）. Meanwhile,their Cort level of each group were（ 312. 10 ±10. 61）,（ 333. 15 ± 15. 73）,（ 341. 13 ± 19. 25）,（ 342. 59 ± 25. 96） and（ 314. 84 ±16. 76） ng / m L,and the 0. 05,0. 1 and 0. 2 μg / m L groups were significantly increased（ P〈0. 05）. Furthermore,the T4 level of male groups exposed to arsenic for 194 days were（ 23. 06 ± 5. 54）,（ 33. 18 ± 5. 29）,（ 34. 23 ± 4. 15）,（ 37. 19 ± 4. 13） and（ 39. 92± 6. 27） ng / m L,respectively. Compared with control group,all experimental group significantly increased（ all P〈0. 05）. Additionally,the T3 level of female group were（ 0. 84 ± 0. 12）,（ 0. 89 ± 0. 14）,（ 0. 83 ± 0. 15）,（ 0. 96 ± 0. 16） and（ 0. 75 ± 0. 11）ng / m L,and 0. 2 μg / m L group obviously increased（ P〈0. 05）. Obvious change of hormone had not been found in other group exposed to subchronic low-dose arsenic（ all P〈0. 05）. Conclusion Subchronic low-dose arsenic exposure have slight endocrine effects on SD rats.