中文摘要：

目的：探讨内皮细胞（endothelial cells，ECS）对血管平滑肌细胞（vascular smooth muscle cells，VSMCs）整合素β1（β1-integrin）表达的影响，为研究血管壁细胞功能提供理论依据.方法：应用血管ECs VSMCs联合培养系统，以Western Blot和细胞免疫荧光技术，检测与ECs联合培养12h后VSMCs的β1-integrin表达和分布变化。结果：与ECs的联合培养，能明显增强VSMCs的β1-integrin蛋白表达，同时诱导VSMCs表面β1-integrin的激活。结论：ECs可能通过渊节VSMCs的β1-integrin表达，从而影响VSMCs的功能。

英文摘要：

Objective： To study the effects of coculture with endothelial cells （ECs） on the expression of β1-integrin in vascular smooth muscle cells （VSMCs）. Methods： Human umbilical veins ECs and VSMCs were isolated. For coculture studies, ECs were grown to confluent on one side of a porous polyethylene terephthalate （PET） membrane. VSMCs were then plated on the opposite side of the membrane and cocultured for 12 hours. Next, the expression of β1-integrin of VSMCs was measured by Western blot and immunofluorescencc microscope. Results： VSMCs cocultured with ECs exhibited a significant increase in the expression of β1-integrin comparing to that of the control, i.e., VSMCs cultured alone. EC-coculture resulted in increased β1-integrin localization to the attached surface of VSMCs. Conclusions： The results suggest that induction of the expression ofVSMC β1-integrin by ECs, may partially influence VSMC function.