中文摘要：

目的：研究5种中药有效部位的抗肿瘤作用及机制。方法：通过细胞病变效应法（CPE）、CCK-8法（CCK-8）和肿瘤病毒抗原激活实验系统,观察5种中药有效部位对肿瘤细胞的细胞毒作用和对肿瘤病毒早期抗原表达的抑制作用。结果：5种中药有效部位在一定的剂量范围内,对Hela细胞、EC-109细胞、MCF-7细胞、Bel-7402这4种不同的肿瘤细胞株均有直接的抑制作用,但抑制率有所差异;同时对肿瘤病毒早期抗原基因的表达也均有明显的抑制作用,抑制率在71.2%-94.4%之间。结论：阻断促癌因素也是防治肿瘤的重要手段和方法。结果提示这些中药有效部位可能是通过细胞毒作用和抑制肿瘤病毒抗原表达和基因复制来达到抗肿瘤的作用。

英文摘要：

Objective： To study the antitumor effects and mechanisms of 5 kinds of Chinese materia medica effective fractions.Methods： With the application of CPE method,CCK-8 assay and tumor virus antigen activation system,Chinese materia medica effective fractions’ cytotoxicity toward tumor cells and inhibitory effects against the expression of tumor virus antigen gene were investigated.Results： 5 kinds of Chinese materia medica effective fractions are all able to inhibit or kill the 4 tumor cell lines including Hela,EC-109,MCF-7 and Bel-7402 cells in a certain dose range,but the inhibitory rates are different.These 5 effective fractions can also inhibit the expression of tumor virus early antigen gene,and the inhibitory rates are between 71.2% and 94.4%.Conclusion： Blocking tumor promoter factor is also the important measure of preventing and treating tumor.The results suggest that these samples may inhibit tumor cell growth through cytotoxicity and inhibiting the expression of tumor virus antigen gene.