中文摘要：

SRK与SCR是甘蓝白交不亲和雌雄性决定因子，两者间相互作具有单倍型特异性。为了探讨HVI／II区域在SRK单倍型特异性及其与SCR互作中的作用，采用重组技术构建甘蓝不同单倍型eSRK（SRKE与SRKF）间的重组体eSRK叫、eSRKE-2和eSRK明，用酵母双杂交系统3检测各eSRK重组体与SCR之间的相互作用。结果表明：SCRE能与eSRKF作用，而不能与eSRKF作用，说明eSRKz、eSRKF属于不同单倍型；SCRE与重组体eSRKE-1、eSRKE-2、eSRK刚均不发生作用，HVI和HVII区域内差异的氨基酸位点共同参与了与SCR的作用；SCRF不能与eSRKE-1、eSRKE-2、eSRKF-3作用，替换HVI／II区域后并不能改变SRK的单倍型。

英文摘要：

Self-incompatibility in Brassica is mediated by allele-specific interactions between stigma-expressed S-locus receptor kinase （SRK） and pollen coat-localized S-locus cysteinerich （SCR） ligands encoded by the S-locus haplotype. To identify amino acid fragments within the SRK extracellular domain （eSRK） that are required for ligand-selective activation, we constructed chi- meric eSRK between two S-locus haplotypes in Brassica oleracea, and identified the interaction between eSRK chimeras and SCRs by yeast two-hybrid system. The results showed that SRKE （not chimera） could interact with SURE, and SRKF could interact with SCRF. All of eSRK chimeras could not interact with SCRs. The hypervariable regions, HVI and HVII, were essential for specificity in the SRK-SCR interaction. However, eSRK chimeras could not interact with SURF, although they contained hyper- variable regions come from eSRKF, which should be related with the overall sequence or 3D conformation of the segments deter- mining SI specificity.