中文摘要：

新城疫病毒（NDV）的V蛋白是一种干扰素拮抗蛋白，该蛋白表达量的差异对NDV细胞嗜性的影响是目前的热点问题。为制备NDV V蛋白多克隆抗体，本研究以Class I NDV 9a5b株和Class II NDV ZJ1 P基因为模板扩增V蛋白PNT结构域和V蛋白半胱氨酸富集区（CTD）结构域，分别克隆至pET-28a（＋）载体和pCold TF载体中，获得表达V蛋白或其CTD结构域的重组表达质粒。将重组质粒转化大肠杆菌诱导表达；纯化重组蛋白，并分别免疫小鼠，制备针对Class I和Class II NDV V蛋白的多克隆抗体。利用该多克隆抗体，western blot检测NDV 9a5b、La Sota、ZJ1、Herts/33株感染HeLa和DF1细胞后V蛋白的表达量变化。结果显示制备的多克隆抗体可以用于NDV V蛋白的检测，V蛋白在易感宿主禽源细胞中的表达量明显高于哺乳动物细胞。研究表明NDV V蛋白的表达水平与宿主细胞的种属差异相关。

英文摘要：

The V protein of Newcastle disease virus （NDV） acts as an interferon antagonist and the impact of expression of V protein on NDV cell tropism has become a hot topic. To study the discrepancy expression of V protein in different cells, the N-terminal of phosphoprotein （PNT） and the C-terminal of V protein （CTD） were amplified from the recombinant plasmids containing the P genes of Class I NDV 9a5b and Class II NDV ZJ1 by PCR and cloned into the pET-28a （＋） and pCold TF vector to express in E.coli, respectively. The antisera against Class I and Class II NDV V proteins were prepared by immunizing BABL/c mice with the purified V proteins, respectively. The expression of V proteins in HeLa and DF1 cells infected with NDV 9a5b, La Sota, ZJ1, Herts/33 were detected by western blot using the prepared polyclonal antisera. The results showed that the expression of the V protein in avian cells were higher than that in mammal cells. These findings indicated that the expression of NDV V protein was affected by c