中文摘要：

目的：建立运用HPLC法同时测定葛根芩连汤中8种有效成分（葛根素、甘草苷、黄芩苷、汉黄芩苷、巴马汀、小檗碱、黄芩素、汉黄芩素）的含量。方法：采用反相高效液相色谱法测定,色谱柱：Agilent ZORBAX Eclipse SB-C18（4.6 mm×250 mm,5μm）;流动相：甲酸铵（pH3.0、10 mmol.L-1）-乙腈,梯度洗脱;流速：1 mL.min-1;检测波长：270、360 nm;柱温：25℃;进样量：20μL。结果：葛根素、甘草苷、黄芩苷、汉黄芩苷、巴马汀、小檗碱、黄芩素、汉黄芩素的质量浓度分别在1.12～22.3μg.mL-1（r=0.9990）,1.08～21.7μg.mL-1（r=0.9991）,4.10～82.0μg.mL-1（r0.9990）,1.23～24.6μg.mL-1（r=0.9993）,1.99～39.8μg.mL-1（r=0.9991）,4.05～81.0μg.mL-1（r=0.9993）,2.40～48.0μg.mL-1（r=0.9994）,1.11～22.1μg.mL-1（r=0.9993）范围内与色谱峰面积呈良好的线性关系。平均回收率（n=5）均在95%～105%范围内,RSD均小于3.0%。结论：本方法可快速简便测定葛根芩连汤中8个有效成分的含量。

英文摘要：

Objective：To establish an HPLC method to determine eight active components in Gegen Qinlian Decoction simultaneously.Methods：HPLC,column：Agilent ZORBAX Eclipse SB-C18 column（4.6 mm × 250 mm,5 μm）,mobile phase：HCOONH3（pH3.0,10 mmol·L-1）-CH3CN,flow rate：1.0 mL·min-1,detection wavelength：270 nm and 360 nm,temperature：25 ℃,injection volume：20 μL.Results：The method has a good linearity in the ranges of 1.12-22.3 μg·mL-1（r =0.9990）for puerarin,1.08-21.7 μg·mL-1（r =0.9991）for liquiritin,4.10-82.0 μg·mL-1（r =0.9990）for baicalin,1.23-24.6 μg·mL-1（r =0.9993）for wogonoside,1.99-39.8 μg·mL-1（r = 0.9991）for palmatine,4.05-81.0 μg·mL-1（r =0.9993）for berberine,2.40-48.0 μg·mL-1（r =0.9994）for baicalein,1.11-22.1 μg·mL-1（r =0.9993）for wogonin.The average recoveries（n=5）of eight active compounds were 95%-105 % with RSDs of recoveries（n=5）were less then 3.0% Conclusion：：The method for determination of 8 active compounds in Gegen Qinlian Decoction,this method can be used for rapid and convenient evaluation of the quality of Gegen Qinlian Decoction.