中文摘要：

利用PCR技术扩增河川沙塘鳢（Odontobutis potamophila）、鸭绿沙塘鳢（Odontobutis yaluensis）、中华沙塘鳢（Odontobutis sinensis）、葛氏鲈塘鳢（Perccottus glenii）及尖头塘鳢（Eleotris oxycephala）的线粒体D—loop区并测序，获得河川沙塘鳢、鸭绿沙塘鳢、中华沙塘鳢、葛氏鲈塘鳢及尖头塘鳢线粒体D—loop区全序列分别为853bp、853bp、1028bp、852bp、848bp，结合GenBank中平头沙塘鳢（Odontobutis platycephala）、刺盖塘鳢（Eleotris acanthopoma）、黑体塘鳢（Eleotris melanosoma）、褐塘鳢（Eleotris fusca）、中华乌塘鳢（Bostrychus sinensis）、云斑尖塘鳢（Oxyeleotris marmorata）、溪鳢（Rhyacichthys aspro））等7种虾虎鱼类同源的D—loop区序列，分析了这12种虾虎鱼类的系统发育关系。所使用的同源序列长度为832—846bp，其中保守位点452个，变异位点405个，简约信息位点258个，单态位点145个，转换／颠换平均值为0．8。基于Kimura2-parameter模型的12种虾虎鱼类遗传距离范围为0．043～0．312，在Kimura2-parameter模型构建的邻接树和非加权配对法系统树中，河川沙塘鳢、鸭绿沙塘鳢、中华沙塘鳢、平头沙塘鳢聚为一大支，刺盖塘鳢、黑体塘鳢、褐塘鳢、尖头塘鳢、溪鳢、葛氏鲈塘鳢聚为另一支。

英文摘要：

To investigate the molecular phylogeny relationship of Gobioidei species fish, D-loop of five speciesfish （Odontobutis potamophila, Odontobutis yaluensis, Odontobutis sinensis, Perccottus glenii and Eleotris oxycephala） were cloned by PCR methods. PCR amplification products of complete D-loop gene fragments from five species fish were 853 bp , 853 bp , 1 028 bp , 852 bp and 848 bp , respectivelyl Together with homologous D-loop sequences of seven species （Odontobutis platycephala, Eleotris acanthopoma, Eleotris melanosoma, Eleotris fusca , Bostrychus sinensis , Oxyeleotris marmorata and Rhyacichthys aspro ）from GenBank, D-loop sequences of twelve species fish were analyzed by MEGA 4.0 software. The results showed that mitochondrial DNA D-loop sequences were 832-846 bp , of which 452 sites were conserved, 405 sites were variable, 258 sites were parsimony informative, 145 sites were singleton sites and the average transitionsal/transversional was 0. 8. Kimura 2-parameter model-based pairwise genetic distances within twelve Gobioidei species were analyzed, and each putative species ranged from 0. 043 -0. 312. And based on phylogenetic trees （N J） and （UPGMA）analysis, the results showed that O. potamophila, 0. yaluensis , 0. sinensis and 0. platycephala belonged to the same branch, E. acanthopoma , E. melanosoma, E. fusca , E. oxycephala , R. aspro and P. glenii belonged to another branch.