中文摘要：

将人工合成的5个相连的XRE（Xeobiotic response element）和minimal CMV（Human cytomegalovirus）启动子，插入载体pEGFP-1，构建得到载体pXRE5-EGFP，以pXRE5－EGFP转染人胃癌细胞SGC－7901，经过筛选得到一株受2，3，7，8－四氯代二苯并二噁英（2，3，7，8－tetrachlorodibenzo—p－dioxin，TCDD）诱导表达绿色荧光蛋白的细胞系XRE5－SGC－7901，将XRE5－SGC－7901细胞株暴露于不同浓度TCDD（0、250、500pg·mL^-1）中，发现TCDD暴露组XRE5-SGC-7901细胞中绿色荧光蛋白的表达量显著升高，且与TCDD暴露浓度呈正相关，新构建的细胞株具有二噁英类化合物诱导表达绿色荧光蛋白的功能。

英文摘要：

Five joined XRE fragments and minimal CMV （Human cytomegalovirus） promoter were inserted into pEGFP-1 to generate a new vector pXRE5-EGFP. When human gastric cancer cell line SGC-7901 was stably transfected with this construct, the recombinant cell line XRE5-SGC-7901 expressed enhanced green fluorescent protein （EGFP） following stimulation with 2,3,7,8-tetrachlorodibenzo-p-dioxin （TCDD）. After exposed to different concentrations of TCDD （0, 250, 500pg·mL^-1）, the cell line expressed EGFP in dose-dependent manners. These results suggested that XRE5-SGC-7901 was a useful bioassay system for dioxin-like chemicals.