中文摘要：

目的：探讨大鼠心肌梗死（MI）后肿瘤坏死因子-α（TNF-α）表达在室性心律失常发生中的作用。方法：采用液氮冷冻法建立大鼠MI模型。将60只雄性大鼠随机分为3组：MI组（20只）、假手术组（Sham组,20只）和TNF-α螯合剂组（rhTNFR：Fc组,20只）。在MI模型建立30d后,运用程序电刺激方法,观察诱发室性心律失常的发生情况,同时用Western Blot和激光共聚焦显微镜检测TNF-α蛋白表达水平。结果：与Sham组比较,MI组大鼠心肌组织TNF-α表达显著增加（P〈0.05）,室性心律失常诱发率亦明显增高（P〈0.05）;与MI组比较,rhTNFR：Fc组TNF-α表达显著减少（P〈0.05）,室性心律失常诱发率亦明显降低（P〈0.05）。结论：MI后大鼠心肌组织TNF-α表达明显增加,大量TNF-α表达与MI后室性心律失常的发生密切相关。

英文摘要：

Objective：To investigate the effect of TNF-α expression on ventricular arrhythmias in rats with myocardial infarction.Method：Sixty male Wistar rats were randomly divided into three groups：Sham group （n=20）,MI group （n=20） and rhTNFR：Fc group （n=20）.Rats with MI were established by liquid nitrogen cryoinjury method.Programmed electrical stimulation was imposed on the hearts to induce ventricular arrhythmia 30 days after myocardial infarction.Protein expression level of TNF-α was detected by western blot and laser scanning confocal 30 days after myocardial infarction.Result：Compared with Sham group,the protein expression levels of TNF-α were significantly increased and the incidence of ventricular arrhythmias was significantly higher in MI group （P〈0.05）.Compared with MI group,the protein expression levels of TNF-α expression were significantly reduced and the incidence of ventricular arrhythmias also significantly lower in rhTNFR：Fc group （P〈0.05）.Conclusion：There is relationship between TNF-α expression and ventricular arrhythmias in rats with MI.