中文摘要：

解淀粉芽孢杆菌（Bacillus amyloliquefaciens）JK-JS3菌株分离自马尾松林,对松材线虫（Bur-saphelenchus xylophilus）具有较高的杀线活性。为进一步开发和应用该菌株,以松材线虫为测试靶标,对JK-JS3菌株分泌杀线活性物质的适宜培养基和培养条件进行了研究。结果表明：不同碳源组分的培养基对JK-JS3菌株分泌杀线活性物质无显著影响,而不同氮源对其有显著影响,其中以氮源为5g/L牛肉膏和6g/L蛋白胨组成的培养基所获培养滤液的杀线活性最高,为实验室条件下菌株分泌杀线活性物质的最适培养基;JK-JS3菌株的最佳培养条件为初始pH=7、培养温度为37℃和无光照振荡培养;该菌株分泌杀线活性物质较理想的培养时间为10d。

英文摘要：

Bacillus amyloliquefaciens strain JK-JS3 was isolated from the needles of Pinus massoniana,which demonstrated with high nematicidal activity on pine wood nematode Bursaphelenchus xylophilus.The culture conditions of strain JKJS3 were studied in the lab.The results showed there were no significant differences in nematicidal effects on B.xylophilus by metabolic materials secreted from JK-JS3 under media with different carbon sources,but the significant differences were observed in those with different nitrogen sources.The best nitrogen sources of the culture media is the mixture of beef extract 5 g/L and peptone 6 g/L.The optimal culture condition for secreting nematicidal materials by JK-JS3 is shaking cultured the media（pH=7） at 37 ℃ without light.The obvious correlation between the culture time and the nematicidal activity is observed and the optimal culture time is 10 days.The study may provide information for controlling the pine wood nematode by the strain JK-JS3 and its nematicidal materials in future.