中文摘要：

从多个矿坑、煤堆废水中富集中度嗜热浸矿菌,并在搅拌反应器中经过两年的长期驯化,获得能耐受高矿浆浓度和具有抗砷性的浸矿混合菌.对浸矿混合菌进行耐高矿浆浓度的驯化,保持矿浆浓度为200g/L,改变两种金矿的配比,提高驯化体系中砷含量,进行耐砷性驯化.用驯化的浸矿混合菌对难处理金矿进行生物氧化预处理,矿浆浓度为200g/L,砷含量为8g/L,14d时菌体浓度最大,达2×109mL-1,22d时砷浸出率为59.93%.采用限制性酶切片段长度多态性分析对浸矿14和22d时的微生物多样性进行分析,发现存在Sulfobacillus sp.,Acidithiobacillus caldus,Ferroplasma the rmophilum和Pseudomonas sp..

英文摘要：

A moderate thermophilic consortium was enriched from acid mine drainage and coal mine wastewater samples collected in China. After adaptation to gradually increased pulp density of arsenic-beating refractory gold ore and concentration of arsenic by serial sub-culturing for about two years, its tolerance ability was up to 200 and 8 g/L, respectively. After that, the consortium was employed to treat arsenic-bearing refractory gold ore to evaluate its ability and potential for industrial application. The results indicated that the maximum cell density was 2x109 mL-1 （the 14th day） during the whole process and arsenic extraction rate reached up to 59.93% in 22 d. Microbial diversity monitored by restriction fragment length polymorphism suggested that the consortium mainly consisted of four species, Sulfobacillus sp., Acidithiobacillus caldus, Ferroplasma thermophilum and Pseudomonas sp..