中文摘要：

目的探讨孕期维生素D缺乏对SD大鼠生殖功能的影响。方法选用8周龄,体质量200~220 g的雌性SD大鼠,共24只。随机分为对照组和维生素D缺乏组,分别饲喂正常饲料及维生素D缺乏饲料。喂养4周后进行合笼,以精子涂片发现精子的当天作为受孕的第1日,孕中期（P11）进行心脏采血,采用液相色谱串联质谱法（liquid chromatography-tandem mass spectrometry,LC-MS-MS）检测孕期维生素D水平,孕鼠分娩后分别记录活胎数及死胎数。结果对照组雌鼠孕中期25-hydroxyvitamin D[25（OH）D]水平为（22.65±2.50）ng/mL,维生素D缺乏组雌鼠孕中期25（OH）D水平为（6.65±1.86）ng/mL,两组比较差异有统计学意义（P〈0.05）。维生素D缺乏组雌鼠每窝活胎数及死胎数分别为（8.90±4.47）只和（2.10±1.93）只,而对照组雌鼠的平均每窝活胎数及死胎数分别为（12.63±2.98）只和（0.00±0.00）只。维生素D缺乏组孕鼠分娩后,其每窝活胎数及死胎数与对照组相比差异均有统计学意义（P〈0.05）。结论维生素D缺乏使大鼠的生殖能力明显下降。

英文摘要：

Objective To study the effect of vitamin D deficiency during pregnancy on the function of reproduction of SD rats. Methods Twenty-four 8-week-old SD female rats,weighting 200-220 g,were divided into two groups randomly： control group and vitamin D deficiency group. The rats in control group were fed control forage,and those in vitamin D deficiency group were fed forage without vitamin D. After 4 weeks,female rats were caged together with male rats. Serum vitamin D levels were measured on the 11 th day of pregnancy by LC-MS-MS,and reproduction experiments were carried out after childbirth. Results The maternal serum vitamin D levels of vitamin D deficiency group were significantly lower than those of the control group. The serum vitamin D levels in control group were（ 22. 65 ± 2. 50） ng / mL,and those in vitamin D deficiency group were（ 6. 65 ± 1. 86） ng / mL（ P 0. 05）. In the vitamin D deficiency group,the number of live births per litter was significantly decreased（ 8. 90 ± 4. 47 in vitamin D deficiency group and 12. 63 ± 2. 98 in control group）. By contrast,the number of stillbirths per litter was significantly increased（ 2. 10 ± 1. 93 in vitamin D deficiency group and 0. 00 ± 0. 00 in control group）. Conclusion Vitamin D deficiency during pregnancy can impair reproductive function.