欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
目的建立了液相色谱-离子阱质谱联用定性测定48种磺胺类、喹诺酮类,四环素类、氯霉素类、大环内酯类和抗真菌类的方法,并用于中成药和保健食品非法添加的检测。方法样品通过甲醇超声振荡提取,其液相色谱分离采用Agilent Eclipse Plus C18柱,流动相A为0.2%甲酸,流动相B为甲醇,梯度洗脱;使用Ion-trap LC/MS分析模式,电喷雾离子源、正离子模式检测。通过与标准谱库中保留时间和多级质谱图的数据进行比对,定性确定样品中添加的抗生素种类。结果 10批中成药中有1批检出氧氟沙星,25批保健食品中有7批检出磺胺甲噁唑。结论本方法解决了5对同分异构体的分离和质谱鉴定问题,并实现同一液质系统内分析多种结构类型的化合物,特别适合中成药和保健食品中违禁抗生素类化学药品的测定。
英文摘要：
AIM To use Ion-trap LC / MS to qualitatively analyze forty-eight antibiotics including sulfanilamides,quinolones,tetracyclines,chloram phenicols,macrolides and antifungal agents.The method has been applied to the illegal acts in traditional Chinese patent medicines and health foods.METHODS The test solutions of samples were prepared by combining ultrasonic extraction and disintegration.Separation was performed on an Agilent Eclipse Plus C18 column with the binary mobile phase consisting of 0.2% formic acid（ solvent A） and methanol（ solvent B） in a gradient elution mode,Ion-trap LC / MS analytic pattern and the electrospray ionization source in positive ion mode.Comparing with retention time and spectrums of references in library set up,the target compounds in sample were screened and identified.RESULTS Ofloxacin was found in one out of ten samples and sulfamethoxazole was found in seven samples out of twenty-five batches of health foods.CONCLUSION The method has solved the problem of separation of five pairs of isomers and qualitative analysis by MS.Several structure kinds of compounds can be analyzed in one system.It is quite suitable for confirmation of illegally added antibiotics drugs in medicines and health foods.
同期刊论文项目
同项目期刊论文
Label-Free Quantitative Proteomic Analysis of Abscisic Acid Effect in Early-Stage Soybean under Floo
CYTOKININ OXIDASE/DEHYDROGENASE4 Integrates Cytokinin and Auxin Signaling to Control Rice Crown Root
In vivo monitoring of nitric oxide in tobacco leaves by a polyNi(II)-tetrasulfonated phthalocyanine/
Dispersive Microextraction Based on “Magnetic Water” Coupled to Gas Chromatography/Mass Spectrometry
Facile synthesis of magnetic one-dimensional polyaniline and its application in magnetic solid phase
Rapid determination of polycyclic aromatic hydrocarbons in environmental water based on magnetite na
Facile one-pot synthesis of a aptamer-based organic-silica hybrid monolithic capillary column by &qu
Quantification of 5-Methylcytosine and 5-Hydroxymethylcytosine in Genomic DNA from Hepatocellular Ca
A novel amperometric adenosine triphosphate biosensor by immobilizing graphene/dual-labeled aptamers
Isotope labelling - paired homologous double neutral loss scan-mass spectrometry for profiling of me
Electrospun polystyrene/oxidized carbon nanotubes film as both sorbent for thin film microextraction
TiO2-Based Solid Phase Extraction Strategy for Highly Effective Elimination of Normal Ribonucleoside
Determination of eight illegal drugs in human urine by combination of magnetic solid-phase extractio
Tomato SlDREB gene restricts leaf expansion and internode elongation by downregulating key genes for
Profiling of Thiol-Containing Compounds by Stable Isotope Labeling Double Precursor Ion Scan Mass Sp
Arabidopsis Plastid AMOS1/EGY1 Integrates Abscisic Acid Signaling to Regulate Global Gene Expression
Preparation of a Novel Amino-Phosphate Zwitterionic Stationary Phase for Hydrophilic Interaction Chr
Preparation of magnetic hydroxyapatite clusters and their application in the enrichment of phosphope
Determination of Endogenous Brassinosteroids in Plant Tissues Using Solid-phase Extraction with Doub
Coupling frontal elution paper chromatography with desorption corona beam ionization mass spectromet
In vivo monitoring of oxidative burst on aloe under salinity stress using hemoglobin and single-wall
Simultaneous Analysis of Multiple Endogenous Plant Hormones in Leaf Tissue of Oilseed Rape by Solid-
Highly sensitive profiling assay of acidic plant hormones using a novel mass probe by capillary elec
Quick, easy, cheap, effective, rugged and safe method with magnetic graphitized carbon black and pri
Dispersive microextraction based on magnetic polypyrrole nanowires for the fast determination of pes
Real-time monitoring of auxin vesicular exocytotic efflux from single plant protoplasts by amperomet
A highly sensitive nitric oxide biosensor based on hemoglobin-chitosan/graphene-hexadecyltrimethylam
Using pollen grains as novel hydrophilic solid-phase extraction sorbents for the simultaneous determ
“Old” metal oxide affinity chromatography as “novel” strategy for specific capture of cis-diol-conta
Determination of Oxidation Products of 5-Methylcytosine in Plants by Chemical Derivatization Coupled
Facile fabrication of reduced graphene oxide-encapsulated silica: A sorbent for solid-phase extracti
Facile Preparation of SiO2/TiO2 Composite Monolithic Capillary Column and Its Application in Enrichm
Facile preparation of organic-silica hybrid monolith for capillary hydrophilic liquid chromatography
Mutagenic and Cytotoxic Properties of Oxidation Products of 5-Methylcytosine Revealed by Next-Genera
Magnetic solid-phase extraction using magnetic hypercrosslinked polymer for rapid determination of i
Methods of sample preparation for determination of veterinary residues in food matrices by porous mo
Preparation of magnetic poly(diethyl vinylphosphonate-co-ethylene glycol dimethacrylate) for the det
Gibberellin Regulates the Arabidopsis Floral Transition through miR156-Targeted SQUAMOSA PROMOTER BI
A selective pretreatment method for determination of endogenous active brassinosteroids in plant tis
Facile synthesis of magnetic molecularly imprinted polymers and its application in magnetic solid ph
A facile approach for the polymer grafting of silica based on tandem reversible addition fragmentati
A simple sample preparation approach based on hydrophilic solid-phase extraction coupled with liquid
In-syringe dispersive solid phase extraction: a novel format for electrospun fiber based microextrac
Magnetic solid phase extraction coupled with in situ derivatization for the highly sensitive determi
期刊信息
位置：