中文摘要：

基于细胞Raf/MEK/ERK信号通路与病毒复制的关系，应用Western印迹检测p-ERK1/2蛋白的表达、用终点滴定法测定病毒增殖量（TCID50），以及观察感染细胞的细胞病变效应（CPE）等，揭示单纯疱疹病毒Ⅱ型（HSV-2）复制与ERK通路的关系.结果表明，HSV-2的复制可引起细胞ERK通路的活化；用U0126预先抑制ERK通路的活化，或用特异性siRNA敲减MEK1/2基因的表达可显著地抑制病毒复制.提示ERK信号通路以及MEK1/2蛋白对HSV-2的复制具有重要的作用.该研究对进一步阐明细胞ERK通路各激酶蛋白在病毒复制中的作用机制、寻找抗病毒作用靶标等奠定了良好的基础.

英文摘要：

Herpes simplex virus type 2（HSV-2） is an important pathogen that causes sexually transmitted genital infections worldwide.Now no specific therapeutic drugs or preventive vaccines are available.Recently,it has been reported that Raf/MEK/ERK（ERK） signal pathway is relevant with virus amplification.MEK1/2 are the key components of the ERK pathway.Thus,we investigated whether MEK1/2 and its associated ERK pathway were required for virus replication.By Western blot analysis,end-point assay for virus titer,and microscopic observation of cytopathic effect（CPE） of viral infection,we demonstrated that ERK pathway was activated within the cells where HSV-2 replicated.Moreover,the inhibition of the ERK signaling pathway by MEK1/2 inhibitor U0126 significantly impaired HSV-2 progeny production,which correlated with the suppression of p-ERK1/2 expression in infected cells.Furthermore,the knockdown of MEK1/2 by specific small interfering RNA（siRNAs） also obviously blocked HSV-2 replication.The results indicated that MEK1/2 and its associated ERK pathway play an essential role in the replication of HSV-2.Silencing the expression of MEK1/2 and perturbing the ERK signaling pathway exhibit an evident anti-HSV-2 effect,suggesting that it can serve as a potential target for a therapeutic drug.