中文摘要：

目的：观察血糖水平对缺氧缺血新生大鼠体质量和脑体比的影响。 方法：实验于2002—10／2004—02在上海交通大学医学院附属新华医院动物实验中心完成。选择7日龄SD新生大鼠485只，随机数字表法分为10组，分别为正常组51只，不予任何处理；假手术组37只，予颈部作一切口，分离右颈总动脉后不予结扎，再缝合颈部切口；单纯低血糖组47只，于缺氧缺血前禁食12h，其后不予缺氧缺血；缺氧缺血组51只，仅给予缺氧缺血；缺氧缺血前低血糖组49只，在缺氧缺血前禁食12h后再予缺氧缺血；缺氧缺血后低血糖组44只，在缺氧缺血后禁食12h；缺氧缺血前轻度高血糖组49只，缺氧缺血后轻度高血糖组47只，分别于缺氧缺血前或后2h始皮下注射250g／L葡萄糖（1．5g／kg），共2次，每次间隔60min；缺氧缺血前重度高血糖组52只，缺氧缺血后重度高血糖组58只，分别于缺氧缺血前或后2h始皮下注射250g／L葡萄糖（1．5g／kg），共3次，每次间隔45min。后7组又分缺氧缺血后2，24，48，72h，7d5个时相点，每个时相点7～10只。于缺氧缺血即刻测定各组新生大鼠尾部血糖值，并分别在缺氧缺血即刻和各时相点称体质量，根据所测体质量和缺氧缺血即刻测得体质量之差评估各组体质量增长情况和断头后脑体比。 结果：纳入动物485只，均进入结果分析。成功制备了各组模型。正常新生大鼠的体质量和脑体比随日龄增加分别呈上升和下降趋势。所有缺氧缺血组新生大鼠在缺氧缺血后24h与正常组在体质量增长和脑体比差异均呈显著性或非常显著性意义（P〈0．05或0．01），尤以缺氧缺血前或后低血糖组的体质量增长最为缓慢，脑体比最高。缺氧缺血前或后合并轻或重高血糖对大鼠的体质量增长则无明显影响，缺氧缺血前重度高血糖组的脑体比与正常组比较差异无显著性意?

英文摘要：

AIM： To explore the influence of different blood glucose levels on the body mass and the ratio of brain weight to body mass （B/D） in neonatal rats with hypoxic ischemia （HI）. METHODS： The experiment was conducted in the animal experimental center of Xinhua Hospital affiliated to Medical Collage, Shanghai Jiao Tong University from October 2002 to February 2004. 485 seven-day-old neonatal SD rats were randomly divided into 10 groups： normal group （n=51） without any treatment; sham operation group （n=37）, which was made an incision on neck without ligation after separated the right common carotid artery; hypoglycemia group （n=47）： fasting for 12 hours before HI; HI group （n=51） given HI; hypoglycemia before HI group （n=49）： fasting for 12 hours before HI and following HI; hypoglycemia after HI group （n=44）： fasting for 12 hours after HI; mild hyperglycemia before HI group （n=49） and mild hyperglycemia after HI group （n=47）, which were given twice subcutaneous injection of 250 g/L glucose （1.5 g/kg） with an interval of 60 minutes before or after HI, respectively; higher hyperglycemia before HI group （n=52） and higher hyperglycemia after HI group （n=58）, which were given subcutaneous injection of 250 g/L glucose （1.5 g/kg） for three times with an interval of 45 minutes before or after HI, respectively. The latter 7 groups were subdivided into 5 subgroups according to time phases at 2, 24, 48, 72 hours and 7 days after HI with 7-10 rats at each time point. Blood glucose level on the tail of neonatal rats was determined immediately before HI. The body mass of all rats were weighted immediately before HI and the increase of the body mass and B/D were evaluated based on the difference of the body mass and the body mass determined immediately before HI. RESULTS： All 485 rats enrolled the result analysis. Neonatal rat models were successfully prepared. There were an increase in the body mass and a decrease in B/D with the increase of day. The signific