中文摘要：

本研究以原核表达的融合型的重组蛋白pET32a（＋）-P（狂犬病病毒HepFlury株P蛋白）免疫6-8周龄的BALB/c小鼠,取脾细胞和SP2/0按常规杂交瘤技术进行细胞融合,经间接免疫荧光方法（IFA）筛选及对mAb进行初步鉴定。经3次亚克隆后获得1株稳定分泌表达的杂交瘤细胞株5G8。

英文摘要：

The fusion protein（pET32a（＋）-P）was purified by TrapFF crude,6-8-week-old BALB/c mice were immunized with the protein mixed with adjuvants before the splenic cells of immunized mice were fused with SP2/0 myeloma cells.Indirect fluorescence antibody detection assay was used to screen hybridoma cells for the specific monoclonal antibodies.One hybridoma（5G8） producing monoclonal antibody were obtained after three rounds of subcloning.