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中文摘要：
目的：建立同时测定朝鲜淫羊藿中新绿原酸、绿原酸、隐绿原酸和金丝桃苷含量的超高效液相色谱（UPLC）方法。方法：采用Acquity BEH C18色谱柱（2.1mm×50mm,1.7μm）;以0.1%乙酸-甲醇为流动相,梯度洗脱;流速：0.4mL/min;柱温：45℃;检测波长为325nm和353nm。结果：新绿原酸、绿原酸、隐绿原酸和金丝桃苷分别在0.576-57.600、1.806-180.600、1.056-105.600和1.530-153.000μg/mL内具有良好的线性关系（R2〉0.9996）;平均回收率分别为96.54%,103.60%,98.61%和102.10%。结论：该方法测定朝鲜淫羊藿中新绿原酸、绿原酸、隐绿原酸和金丝桃苷的含量,具有简便快速、可靠的优点。
英文摘要：
Objective： To develop an UPLC method for the simultaneous determination of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid and hyperin in Epimedium koreanum Nakai.Methods： Chromatography was performed on a Acquity BEH C18 column（2.1mm×50mm,1.7μm） with the mobile phase of methanol and water containing 0.1% acetic acid;Flow rate was 0.4 mL/min and column temperature was 45℃;The detection wavelength was set at 260nm and 325nm.Results： The correlation coefficient between concentration and chromatographic peak area of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid and hyperinwas over 0.9996 in the range of 0.576-57.600,1.806-180.600,1.056-105.600 and 1.530-153.000μg/mL,respectively.The average recoveries of the four compounds were 96.54%,103.60%,98.61% and 102.10%,respectively.Conclusion： The method is convenient and feasible for determination of the content of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid and hyperin in Epimedium koreanum Nakai.
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