中文摘要：

用制备并纯化的鸡p15^INK4B蛋白免疫BALB／c小鼠，制备了p15^INK4B蛋白的单克隆抗体，并进行了鉴定。结果表明：2株分泌单克隆抗体的杂交瘤细胞株105和3F5分别属于IgG1和IgM抗体亚类；培养上清的效价分别为1：820和1：680，腹水的效价分别为1：4×10^6和1：7×10^5；2株单克隆抗体亲和力常数分别为3．13×10^9L/mol（1C6）和1．24×10^8L／mol（3F5），相对亲和力1C6〉3F5，且具有不同的抗原识别位点；Westem-blot鉴定表明，1C6和3F5这2株单克隆抗体均能与原核表达纯化的和杆状病毒表达的p15^INK4B蛋白结合，而不与其他蛋白结合，说明本研究成功制备了鸡p15^INK4B的单克隆抗体，可作为深入研究鸡p15^INK4B蛋白的功能提供特异的抗体材料。

英文摘要：

Preparation of monoclonal antibody （McAb） against chicken p15^INK4B （p15） protein was carfled out for the function research of pl5.The purified p15 protein as antigen immunized BALB/c mice. Two hybridoma, 1C6 and 3F5, stably secreting McAb were produced by fusion of SP2/0 myeloma cells and spleen cells of syngeneic mice which had been previously immunized by p15 protein. Their titers in cell culture supematant were 1 ： 820 and 1 ： 680 respectively, titers in ascites were 1 ： 4 ×10^6 and i ： 7×10^5 respectively, and affinity constants were 3.13 × 10^9 and 1.24 ×108 L/mol respectively. The monoclonal antibodies were of high affinity and different antigen binding epitope, and they could specifically bind to p15 protein purified from prokaryotic expressing product and expressed in BAC-TO-BAC Baculovirus Ex- pression Systems but not other cell proteins. All of these show that the McAb against p15 was successfully prepared.