中文摘要：

为了探讨基因Ⅳ型戊型肝炎病毒开放阅读框3（ORF3）表达的蛋白（pORF3）影响靶细胞基质金属蛋白酶（MMP）表达的分子机制，试验运用蛋白芯片技术对比分析了稳定表达pEGFP—ORF3和pEGFP的L02细胞中基质金属蛋白酶表达谱，筛选出表达水平上调／下调的基质金属蛋白酶。结果表明，在稳定表达pEGFP—ORF3的L02细胞中，TIMP1、TIMP-2和TIMP-4表达水平均降低。本试验结果为戊型肝炎病毒pORF3对L02细胞中基质金属蛋白酶相关蛋白表达影响的分子机制研究提供了理论依据。

英文摘要：

To discuss the mechanism how open reading frame 3 （ORF3） protein of genotype IV hepatitis E virus （HEV） affected the expression of matrix metalloproteinase （MMP） in target cells, Human Matrix Metalloproteinase Antibody Array was used to compare and analyze the expression levels of matrix metalloproteinase in stable cell lines that expressed or did not express HEV ORF3, screening the matrix metalloproteinases which were down-regulated or up-regulated. The results indicated that the expressions of TIMP-1 ,TIMP-2 and TIMP-4 in stable L02 cell line with expression of pEGFP-ORF3 were down-regulated, and which provided important information for explaining the molecular mechanism through which HEV pORF3 affected the expression of protein associated with matrix metalloproteinase in target cells.