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中文摘要：
为减少梗丝中的木质素含量,改善梗丝品质,提高其在卷烟中的应用水平,对漆酶降解梗丝木质素的4个影响因素（处理温度、处理时间、漆酶用量和漆酶溶液p H）进行单因素试验和响应面法工艺优化,对处理前后的梗丝进行电镜扫描,并将其制成单料烟进行感官评价。结果表明：1优化工艺条件为处理温度45.0℃,处理时间3.0 h,漆酶用量20.0 U/g,漆酶溶液p H 6.0,木质素理论降解率56.68%,实测值55.05%。2漆酶处理后的梗丝表面更加光滑,毛突减少。3与对照相比,试验卷烟的香气质改善,香气量提高,余味改善,杂气、刺激性、浓度降低,而劲头、燃烧性及灰色无明显变化。因此,采用该方法得到的漆酶降解梗丝木质素工艺条件是可靠的,可为梗丝在卷烟中的应用提供方法参考。
英文摘要：
In order to reduce the lignin content in cut stems,to improve the quality of cut stems and to increase their proportion in cigarette blends,single factor experiments were conducted to study the effects of four factors involved in laccase treatment of cut stems（treatment temperature,treatment time,addition rate of laccase,p H of laccase solution） on the degradation of lignin in cut stems. The degradation technology was optimized by response surface method,the cut stems were analyzed by scanning electron microscope before and after the laccase treatment,and the sensory quality of cigarettes made of the treated and untreated cut stems was panel tested,separately. The results showed that：1） The optimized treatment condition was at temperature 45.0 ℃,treatment time 3.0 h,the addition rate of laccase 20.0 U/g,and p H of laccase solution at 6.0. The calculated degradation rate of lignin was 56.68%,and the measured value was 55.05%. 2）The surface of the treated cut stems by laccase was smoother with less burrs. 3） Comparing with the control,the aroma quality,aroma quantity and aftertaste of the test cigarette smoke were improved,the levels of offensive odor and irritation were reduced,while the strength,combustibility and ash color did not change obviously. In summary,the optimized treatment condition for degrading lignin in cut stems with laccase is reliable,which provides the potential to expand the application of cut stem in cigarette.
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