中文摘要：

目的研究妊娠期甲醛和苯联合染毒对小鼠胚胎发育的影响。方法将妊娠0天的昆明小鼠随机分为对照组、低剂量组、中剂量组、高剂量组,并分别于妊娠第6天开始进行吸入式染毒,连续15 d,甲醛和苯的剂量为国家室内空气标准的0倍、1倍、50倍和100倍。仔鼠出生后,记录正常生产孕鼠数、产仔数、流产孕鼠数及出生24 h后存活的仔鼠数。仔鼠称重后分离肝及脑组织并进行RT-PCR,检测部分脑、肝发育相关基因在组织内的表达情况。取仔鼠肝组织,固定后免疫组化检测IGF-1的表达情况。结果高剂量组的流产孕鼠数高于对照组、低剂量组及中剂量组（P〈0.05）;高剂量组的产仔数、存活数低于其余各组（P〈0.05）;中剂量组、高剂量组仔鼠体重低于对照组和低剂量组（P〈0.05）;高剂量组发育相关基因Peg3和IGF-1的表达强度低于对照组、低剂量组及中剂量组（P〈0.05）。免疫组化结果显示仔鼠肝组织中IGF-1表达降低。结论妊娠期接触甲醛与苯混合气体可导致孕鼠流产率上升、产仔数、仔鼠存活数、仔鼠体重下降,同时也会导致胚胎发育过程中重要基因IGF-1和Peg3表达下调。

英文摘要：

Objective To explore the joint toxic effecs of formaldehyde and benzene on embryos of mice.Methods Pregnant Kuming mice were randomly divided into four groups including control group,low dose group,middle dose group and high dose group.Mice were allowed to inhale the mixture of gas for 15 days continually since 6dpc.The dose of formaldehyde and benzene were 0,1,50 and 100 times of national standard.The number of normal pregnancy,abortion,the number of newborns and survival of 24 hours after birth were recorded.The newborns were weighed as well as their brains and livers.RNA was extracted from parts of liver and brain in order to operate RT-PCR and find out the expression of development related genes.Livers were fixed and used to detect the expression of IGF-1 through immunohistochemistry.Results The number of abortion in high dose group was more than that of other groups while the number of newborn and the survival was less（P＆lt;0.05）.The weight of newborn in high dose group and middle dose group were less than that in control group and low dose group（P＆lt;0.05）.The expression of development related genes,IGF-1 and Peg3,decreased in high dose group（P＆lt;0.05）.The results of immunohistochemistry also showed that the low-expression of IGF-1.Conclusion The last contact of formaldehyde and benzene mixture gas during pregnancy would lead to abortion,the decrease of the number of newborn,survival of 24 hours after birth and the weight of newborn.Meanwhile,the expression of development genes,IGF-1 and Peg3,would also decrease significantly.