中文摘要：

100只艾维因雏鸡随机分成益生素组、新城疫（ND）疫苗免疫组、益生素ND疫苗组和对照组,采用组织石蜡切片及酸性α-醋酸萘酯酶（acid nonspecifica-naphthyl acetate esterase,ANAE）染色法分别测定ND疫苗首次免疫后第0、7、14、28天和第42天各组雏鸡盲肠扁桃体、哈德尔氏腺、十二指肠和回肠派伊尔结T淋巴细胞数量的动态变化,结果发现：益生素ND疫苗免疫雏鸡,ND疫苗单独免疫雏鸡上述免疫组织T淋巴细胞数量于ND疫苗免疫后14 d明显高于未免疫的对照雏鸡和益生素雏鸡;益生素ND疫苗免疫雏鸡又不同程度高于ND疫苗单独免疫雏鸡;益生素单独应用雏鸡上述被检指标高于对照雏鸡。表明益生素可增强雏鸡消化道和呼吸道局部黏膜组织的细胞免疫及对ND疫苗的免疫应答。

英文摘要：

One hundred chicks were randomly divided into probiotics proup（P group）,newcastle disease（ND） vaccine group（V group）,probiotics ND vaccine group（P＋V group） and normal group（N group）.The dynamic changes of T lymphocytes number in the cecal tonsil,gland of Harderian gland,and Peyer′s patch of duodeum and ileum of probiotics-treated chicks vaacinated with ND vaccine were determined using paraffin section and acid nonspecifica-naphthyl acetate esterase（ANAE） staining method 0,7,14,28 and 42 days post vaccination.The results showed that the numbers of T lymophocytes in above immune tissues of P＋V group and V group chicks were singificantly enhanced compared wht N and P groups 14 day after vaccination.The number of T lymphocytes in the cecal tonil,Harderian gland,and Peyer′s patch of duodenum and ileum of probiotics chicks vaccinated with ND vaccine increased as compared to that of chicks vaccinated with ND vaccine only.The number of T lymphocytes in above immune tissues of chicks treated with probiotics increased as compared to that of normal chicks.These results indicate that probiotics can enhance the celiular immune function in gastrointestinal and respiratory local mucosa of chicks and the cellular immune response against ND vaccine.