中文摘要：

目的：通过离体培养脊髓星形胶质细胞,探讨谷氨酸对缝隙连接蛋白43磷酸化的调节机制.方法：实验分为正常对照组、谷氨酸刺激组（10 μmol/L谷氨酸作用24h）、PKC抑制剂组（20 μmol/L Go6976作用24 h）.Western Blot法检测各组星形胶质细胞中P-CX43、P-PKC、P-ERK蛋白的表达;细胞免疫荧光法检测各组星形胶质细胞P-CX43胞膜蛋白的表达.结果：谷氨酸刺激组与正常对照组相比,星形胶质细胞P-CX43、P-PKC蛋白的表达增加（P＜0.05）;在谷氨酸刺激的基础上给予PKC抑制剂作用后P-CX43的表达降低（P＜0.05）.结论：谷氨酸可以通过PKC通路使CX43的磷酸化增加.

英文摘要：

Objective： To explore the mechanism of glutamic acid reyulating CX43 phosphorylation in cultured astrocytes of spinal cord in vitro. Methods： Cultured neonatal SD rat astrocytes were divided into normal control group, glutamic acid stimulation group（ treated with 10/xmol/Glu for 24 h） and PKC inhibitor group. Western Blot assay was used to detect P-CX43, P-PKC, P-ERK protein expression, cell immunofluorescence assay were used to detect the P-CX43 expression in astroeytes membrane. Results： Compared with the normal control cells, glutamie acid treatment group resulted in a significantly increas of the expression of P-CX43, P-PKC in the astroeytes （ P 〈 0.05 ）. After glutamic acid stimulation, PKC inhibitor reduced the expression of P-CX43 （ P 〈 0.05 ）. Conclusion： Glutamie acid can increase the expression of P-CX43 protein through PKC pathway.