中文摘要：

我们使用了 Populus euphratica 奥利夫的胼胝孤立原物，并且越过质膜的 H~+ 流动被调查。为原物隔离的酶的集中例如绗维素酶， pectolyase， macerozyme，半绗维素酶，和山梨糖醇内容，孵化时间是全身的学习的联盟者。原物的高产和生存能力在 6-8 小时孵化 oi P 以后被完成。在包含 1.5% 的酶答案(w:v ) 的 euphratica 胼胝绗维素酶 R-10， 0.1%(w:v ) pectolyase Y-23， 0.2%(w:v ) macerozyme R-10， 0.05%(w:v ) 半绗维素酶和 0.75-0.80 mol.L~(-1) 山梨糖醇。Non-invasively 离子选择微电极技术被用来在 NaCl 的缺席和存在存取质子流动(20 mmol.L~(-1)) 。导致盐的短暂的网 H4 流出在 P 的质膜被观察。euphratica 房间。对到盐忍耐的 NaCl 吃惊和关联的 H~+ 流动反应的移动被讨论。

英文摘要：

We used callus of Populus euphratica Olive to isolate protoplasts, and IT fluxes across plasma membrane were investigated. The concentration of enzymes for protoplast isolation, e.g. cellulase, pectolyase, macerozyme, hemicellulase, and sorbitol content, incubation time were systemically studied. High yield and viability of protoplast was achieved after 6-8 hours incubation of P. euphratica callus in enzyme solution containing 1.5% （w：v） cellulase R-10, 0.1% （w：v） pectolyase Y-23, 0.2% （w：v） macerozyme R-10, 0.05% （w：v） hemicellulase and 0.75M）.80 mol·L^-1 sorbitol. Non-invasively ion selective microelectrode technique was used to access proton fluxes in the absence and presence of NaCl （20 mmol.L-1）. Salt-induced transient net IT effiux was observed in the plasma membrane ofP. euphratica cells. The shift of IT flux response to NaC1 shock and the relevance to salt tolerance were discussed.