中文摘要：

目的 探讨还原型谷胱甘肽（GSH）对阻塞性黄疸大鼠胸主动脉收缩与舒张功能的影响.方法 雄性SD大鼠24只,体重200 ～ 250 g,采用随机数字表法分为4组（n=6）：假手术组（S组）、GSH组、胆总管结扎组（BDL组）和谷胱甘肽＋胆总管结扎组（GSH＋ BDL组）.GSH组和GSH＋BDL组每天经胃灌注GSH 300 mg/kg,连续7d,其他2组给予等容量生理盐水.灌胃结束后1 d BDL组和GSH＋ BDL采用胆道结扎法制备大鼠阻塞性黄疸模型.GSH组和GSH＋ BDL组造模后每天经胃灌注GSH 300 mg/kg,连续7d,其他2组给予等容量生理盐水.于造模后第7天,取血样,测定血清总胆红素（TB）、丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、TNF-α、IL-1β和3-硝基酪氨酸（3-NT）、MDA、NO和GSH水平.随后制备胸主动脉环,分别灌注不同浓度去甲肾上腺素（NE）、乙酰胆碱（Ach）和硝普钠（SNP）,计算最大收缩百分比和最大舒张百分比.另取部分胸主动脉环,去除内皮或灌注一氧化氮合酶抑制剂亚硝基左旋精氨酸甲酯后,灌注不同浓度NE,计算最大收缩百分比.结果 与S组比较,BDL组血清TB、ALT、AST、TNF-α、IL-1β、MDA、3-NT、NO和GSH水平升高,BDL＋ GSH组血清TB、ALT、AST、MDA、GSH和NO水平升高,BDL组和BDL＋ GSH组胸主动脉环对NE诱发的最大收缩百分比降低,对Ach和SNP诱发的最大舒张百分比降低（P＜0.05或0.01）,GSH组上述指标差异无统计学意义（P＞0.05）;与BDL组比较,BDL＋ GSH组血清TB、ALT、AST、TNF-α、IL-1β、MDA、3-NT和NO水平降低,GSH水平升高,胸主动脉环对NE诱发的最大收缩百分比升高,对Ach诱发的最大舒张百分比升高（P＜0.01）,对SNP诱发的最大舒张百分比差异无统计学意义（P＞0.05）.与BDL组去除内皮或经亚硝基左旋精氨酸甲酯灌注的胸主动脉环比较,BDL＋ GSH组去除内皮或经L-NAME灌注的胸主动脉环对NE诱发的最大收缩百分比差异无统计学意义（P＞0

英文摘要：

Objective To evaluate the effects of reduced glutathione （GSH） on contractile and diastolic functions of the thoracic aorta of rats with obstructive jaundice.Methods Twenty-four adult male Sprague-Dawley rats,weighing 200-250 g,were randomly divided into 4 groups （n =6 each） using a random number table：sham operation group （group S）,GSH group,bile duct ligation group （group BDL）,and GSH ＋ bile duct ligation group （group GSH ＋ BDL）.In GSH and GSH ＋ BDL groups,GSH 300 mg/kg was infused via stomach everyday for 7 consecutive days,while the equal volume of normal saline was given in the other two groups.The common bile duct was ligated in the animals on 1 d after the end of intragastric administration in GSH and GSH ＋ BDL groups.In GSH and GSH ＋ BDL groups,after the model was established,GSH 300 mg/kg was infused via stomach everyday for 7 consecutive days,while the equal volume of normal saline was given in the other two groups.On 7th day after the model was extablished,blood samples were collected for determination of the levels of serum total bilirubin （TB）,alanine aminotransferase （ALT） and aspartate aminotransferase （AST）,tumor necrosis factor-alpha （TNF-α）,interleukin-lβ （IL-1β） and 3-nitrotyrosine （3-NT）,malondialdehyde （MDA）,nitric oxide （NO） and GSH.The thoracic aortic rings were obtained and perfused with different concentrations of norepinephrine （NE）,acetylcholine （Ach） and sodium nitroprusside （SNP）.The maximum contraction and dilatation of aortic rings were recorded and the percentage was calculated.Some thoracic aortic rings were obtained again and perfused with different concentrations of NE after removal of the endothelium or after being perfused with L-NAME （nitricoxide synthase inhibitor）.The maximum contraction of aortic rings was recorded and the percentage was calculated.Results Compared with group S,the serum levels of TB,ALT,AST,TNF-α,IL-1β,MDA,3-NT,NO and GSH were significantly increased in group BDL,and the