中文摘要：

这研究的目的是从绿色证实牙齿的肉干细胞( DPSC )的 multilineage 区别能力的目的荧光灯在 DPSC 的 GFP 的转基因的 mice.The 表示也是的蛋白质( GFP )在 differentiation.Methodology 期间观察到 DPSC 从转基因的裸体老鼠的牙齿的肉织物被收获，然后转了到 osteogenic ， adipogenic ，和 chondrogenic media.The 导致的房间的词法描述被显微镜学和组织学的 staining.The expressi 观察

英文摘要：

Aim The aim of this study was to confirm the multilineage differentiation ability of dental pulp stem cells （DPSCs） from green fluorescent protein （GFP） transgenic mice. The expression of GFP in DPSCs was also observed during differentiation. Methodology DPSCs were harvested from the dental pulp tissue of transgenic nude mice, and then transferred to osteogenic, adipogenic, and chondrogenic media. The morphological characterization of induced cells was observed by microscopy and histological staining. The expression of marker genes was measured by RT-PCR. Results The endogenous GFP and multilineage potential of transgenic DPSCs had no influence on each other. Moreover, the results of fluorescence microscopic imaging suggest that there was no significant decline of GFP expression during DPSCs differentiation. Conclusion As the population of GFP labeled DPSCs can be easily identified, this will be a promising method for tracking DPSCs in vivo.