中文摘要：

目的探讨H2O2预处理（HPP）对1-甲基4-苯基吡啶离子（MPP^＋）诱导PC12细胞损伤的保护作用和可能的机制。方法采用4甲基偶氮唑盐法（MTF法）、自动生化分析仪、4，6-二氨基-2-苯基吲哚（DAPI）染色法及免疫印迹（western blot）技术分别检测细胞活力、乳酸脱氢酶（LDH）活性、细胞凋亡及14—3—3蛋白的表达。结果H2O2预处理能够上调14—3—3蛋白表达，增加PCI2细胞活力（MPP^＋组52．46％±6．15％，HPP＋MPP^＋组83．78％±5．84％），抑制LDH的活性[MPP^＋组（37．31±3．99）U／L，HPP＋MPP^＋组（12．49±2．26）U／L]；抑制细胞凋亡（MPP^＋组48．72％±6．68％，HPP＋MPP^＋组17．56％±5．21％）。结论H2O2预处理能减轻MPP^＋对PC12细胞的毒性损伤作用，这种保护作用是通过上调14—3—3蛋白的表达实现的。

英文摘要：

Objective To investigate the protective effects of hydrogen peroxide preconditioning on the injury of PC12 cells induced by M PP^＋ and its mechanisms. Methods MTT^＋ assay, automatic biochemistry analyzer, DAPI stain and western blotting were utilized to determine the cell viability, LDH activity, apoptosis of PC12 cells and expression of 14-3-3 protein respectively. Results Hydrogen peroxide preconditioning could up-regulate 14-3-3 protein expression,increase cell viability （ MPP^＋ group 52.46% ± 6.15%, HPP ＋ MPP^＋ group 83.78% ± 5.84% ）, inhibit LDH activity [ MPP^＋ group （ 37.31 ± 3.99 ） U/L, HPP ＋ MPP^＋ group （ 12.49 ± 2.26 ） U/L ] and cell apoptosis （ MPP^＋ group 48.72% ± 6.68 %, HPP ＋ MPP^＋ group 17.56% ±5.21% ）. Conclusion Hydrogen peroxide preconditioning can protect PC12 cells against MPP^＋ toxicity through up-regulating the expression of 14-3-3 protein.