为了研究北极微藻对不同环境温度的适应性,对分离自北极王湾地区的三种微藻一玛氏骨条藻(Skeletonema marinoi)、小球藻(Chlorella sp.)和衣藻(Chlamydomonas sp.)在不同温度条件下(0℃、4℃和8℃)的生长曲线和抗氧化酶系统进行了测定。结果表明微藻对温度的适应性因种而异:玛氏骨条藻在4℃具有最快生长速率,最高的超氧化物歧化酶(SOD)和过氧化氢酶(CAT)酶活性,及最低的丙二醛(MDA)含量;小球藻的最快生长速率及酶活均在8℃时,MDA含量最低值出现在0℃;衣藻生长情况相差不大,MDA含量的最低值出现在8℃。抗氧化酶系统变化规律也因藻种及温度而异——在0℃与4℃变化时,玛氏骨条藻抗氧化酶系统的三个指标的差异极显著(P〈0.01);在0℃与8℃变化时,玛氏骨条藻与小球藻的SOD活性的差异显著(P〈0.05),其余两个指标差异不显著;衣藻在三个温度下的三个指标差异均不显著。总体而言,三种藻对三个温度的适应性均较好:其MDA的含量均较低,三种藻各自具有独特的生理代谢机制来适应不同的温度环境。
In order to study the adaptability of three species ( Skeletonema marinoi, Chlorella sp. and Chlamydomonas sp. ) to different temperatures, the growth curve and antioxidant enzyme system at different temperatures(0 ℃, d ℃, 8℃ ) were examined. The results showed that the adaptability of three algal species were different at different temperatures : the growth rate, SOD and CAT activities of Skeletonema marinoi was highest and the content of MDA was lowest at 4 ℃ ; the fastest growth rate and activity of enzyme of two Chlorophyta species were at 8 ℃, and the lowest MDA content of Chlamydomonas sp. was at 8 ℃ while that of Chlorella sp. was at 0 ℃. The antioxidant enzyme system were also different among three temperatures--the difference between 0 ℃ and 4℃ of Skeletonema marinoi was very significant ; the difference between 0℃ and 8℃ of SOD activity was significant of Skeletonema marinoi and Chlorella sp. , Chlamydomonas sp. showed insignificant of antioxidant enzyme system at different temperatures. In a word, three algal species have the adaptability at different temperatures.