中文摘要：

目的：观察与微血管系统重建过程密切相关的促血管生成素（Angiopoietin-1，Ang-1）及其受体含免疫球蛋白样环和上皮生长因子样域酪氨酸激酶-2（tymsine kinase that contains immunoglobulin—like loops and epidermal growth factor-similar domains-2，Tie-2）在大鼠基底核脑出血后表达的动态变化。方法：用Ⅶ型胶原酶诱导大鼠脑出血模型，采用HE染色观察大鼠脑组织形态学改变，免疫组化法检测第1、2、4、7、14、21和28d血管生成素Ang-1和其受体Tie-2的表达，计数阳性血管作为观察指标。结果：HE染色显示正常组及假手术组各时间点取材未见血肿及局部明显病理学改变，而模型组第4d血肿周围出现微血管段，而后阳性微血管段表达逐渐持续增多，至第21d大量伸人血肿区；免疫组化研究显示正常及假手术组不同时间点Ang-1和Tie-2表达均未见明显变化，模型组大鼠在脑出血后第2d起Ang-1和Tie-2阳性微血管表达明显多于其他两组，而后表达逐渐升高（P〈0．01），至21d达到高峰，随后开始下降，28d时仍有表达。结论：在脑出血后，损伤区Ang-1及其受体Tie-2的表达上调，可能通过调节血管生成过程而促进脑出血损伤区微血管系统重建。

英文摘要：

Objective： To observe dynamic changes of Angiopoietin-1（Ang-1） and the receptor tyrosine kinase that contains immunoglobulin-like loops and epidermal growth factor-similar domains-2 （Tie-2） expression following intracerebral hemorrhage （ICH）, which were known to be related with angiogenesis in basal ganglia of rat. Method： ICH rats were induced with Collagenase Ⅶ and the changes of brain were observed by HE staining and expression of Ang-1 and the receptor Tie-2 were assayed by immunohistochemistry on the 1st、 2nd、4th、7th、14th、21st and 28th d after the onset, then positive microvessels were counted. Result： No obvious pathology change appeared in brains of normal control and sham or sham group during the experiment, but for model group, microvessels appeared around hematoma on the 4th d, which got denser gradually and extended into hematoma on the 21st d. Immunohistochemistry revealed no change of expression of Ang-1 and Tie-2 in brains of normal control and sham or sham group during the experiment,and in ICH group, the expression of Ang-1 and Tie-2 were observed on the 2nd d,which increased gradually and peaked on the 21st d, then depressed on day 28. Conclusion： Expressions of Ang-1 and its receptor Tie-2 are up-regulated follwing ICH,which may play an important role in the course of microvascular networks reconstruction by promoting angiogenesis.