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S Phase Cell Percentage Normalized BrdU Incorporation Rate, a New Parameter for Determining S Arrest＊
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  • 分类：S858.31[农业科学—临床兽医学;农业科学—兽医学;农业科学—畜牧兽医] O413.1[理学—理论物理;理学—物理]
  • 作者机构：[1]Department of Toxicology, Peking University Health Science Center, Beijing 100191, China, [2]Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Peking University Health Science Center, Beijing 100191, China, [3]Medical and Healthy Analytical Center, Peking University Health Science Center, Beijing 100191, China
  • 相关基金：＊This work was supported by the National Nature Science Foundation of China （Nos. 81370079 and 81001253） and the Beijing Natural Science Foundation （No 7132122）.
作者： ZHAO Peng[1,2], FU Juan Ling[1], YAO Bi Yun[1,2], JIA Yong Rui[3], ZHOU Zong Can[1]
关键词： BRDU, 归一化, 掺入, 细胞, S期, 比例, DNA损伤, 硫酸二乙酯
中文摘要：

<正>In this study,a new parameter,S phase cell percentage(S fraction)normalized BrdU(SFN-BrdU)incorporation rate,was introduced to detect S arrest.The results showed a positive linear correlation between the BrdU incorporation rate and the S fraction in unperturbed 16HBE cells.Theoretical analysis indicated that only S arrest

英文摘要：

In this study, a new parameter, S phase cell percentage （S fraction） normalized BrdU （SFN-BrdU） incorporation rate, was introduced to detect $ arrest. The results showed a positive linear correlation between the BrdU incorporation rate and the S fraction in unperturbed 16HBE cells. Theoretical analysis indicated that only S arrest could result in a decrease in the SFN-BrdU incorporation rate. Additionally, the decrease in SFN-BrdU incorporation rate and the activation of DNA damage checkpoints further demonstrated that S arrest was induced by diethyl sulfate treatment of 16HBE cells. In conclusion, $FN-BrdU incorporation rate can be used to detecting S arrest.

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