中文摘要：

目的动态观察阿霉素肾病大鼠在发生蛋白尿的过程中肾组织血管生成素样蛋白3（ANGPTL3）mRNA表达水平的变化，探讨ANGPTL3在大鼠肾组织中表达与蛋白尿的关系。方法通过激光微分离技术分离阿霉素肾病大鼠肾小球和肾小管，采用实时荧光定量RT-PCR方法分析阿霉素注射后7、14、21和28d肾组织中ANGPTL3 mRNA表达水平的变化。结果（1）阿霉素注射后第14天开始出现大量蛋白尿（P〈0.01），血清白蛋白明显减少（P〈0．01），胆固醇（P〈0．01）和甘油三酯（P〈0．05）明显增高，以上各项指标均在第28天达高峰。（2）通过激光微分离系统成功地分离了大鼠肾组织中的肾小球和肾小管。（3）阿霉素注射后7及14d后，大鼠肾皮质中总ANGFTL3 mRNA表达水平与同一时间点正常对照组比较无明显变化；到第21天时为正常对照组的9．03倍（P〈0．01）；第28天时为正常对照组的3．95倍（P〈0．05），与第21天时相比已呈现下降趋势。（4）阿霉素注射后不同时间点肾小球中ANGPTL3 mRNA表达水平的变化趋势与肾组织总ANGPTL 3mRNA表达水平的变化一致，第7、14天时，肾小球中ANGPTL 3mRNA表达水平与同一时间点正常对照组比较无明显变化；到第21天时为同一时间点正常对照组的2．18倍（P〈0．05）；第28天时为正常对照组的1．90倍（P〈0．05）。（5）阿霉素注射后不同时间点肾小管中ANGPTL3 mRNA表达水平无明显改变，与同一时间点的正常对照组相比均无显著性差异。结论正常大鼠和阿霉索肾病大鼠的肾组织中均有ANGPTL3 mRNA表达。肾组织可以自身合成ANGPTL3；肾病的大鼠肾组织自身合成ANGPTL3增多，且这种改变是发生在肾小球。ANGPTL3可能通过影响肾小球的功能参与肾病蛋白尿的进展过程。

英文摘要：

Objective To study the expression of angiopoietin-like protein 3 （ANGPTL3） mRNA successively in kidney of adriamyein （ADR）-indueed nephrotie rats during the development of proteinuria, and to disclose the possible assosiation of ANGPTL3 with proteinuria. Methods Adriamyeininduced nephrotie rat models were established by a single injection of adfiamyein via the tail vein. Glomeruli and cortex tubuli of rats were dissected by laser microdissection. Real time quantitative RT-PCR was used to study the expression of ANGPTL3 mRNA in kidney of ADR rats at 7, 14, 21 and 28 days successively following adriamycin injection. Results （1） In ADR rats, urinary protein and the level of triglyceride and cholesterol in serum increased significantly at day 14 （P 〈 0.01, 0.05 and 0.01, respectively）, at same time, serum albumin decreased significantly （P〈 0.01）. Changes of these indices reached the peak level at day 28. （2） Glomeruli and cortex tubuli of rats were dissected successfully by laser microdissection. （3） The mRNA of ANGPTL3 in total cortex of kidney in ADR rat increased significandy at day 21 （P 〈 0.01） and at day 28 （P 〈 0.05） compared to that of control. （4） The tendency of ANGPTL3 mRNA expression in glomendi of ADR rats was as the same as that in total cortex. ADR rats had a higher expression level of ANGPTL3mRNA in glomendi at day 21 and day 28 compared to that of control （both P 〈 0.05）. （5） There was no signiticandy change of ANGPTL3 mRNA expression in cortex tubuli following adriamycin injection. Conclusions ANGPTL3 mRNA can be expressed in the kidneys of normal and ARD rats. The expression of ANGPTL3 mRNA increases in glomendi of ADR rats during the development of proteinuria.