中文摘要：

目的：探讨原蛋白转化酶（PCs）在转化生长因子β1（TGFβ1）抑制HBV复制效应中的作用。方法：常规培养的HepG2．2．15细胞加2μg／L或5μg／L重组TGFβ1或／和20μmol/L PC抑制剂作用18h，收集细胞提取细胞总RNA和HBV衣壳DNA，采用荧光定量PCR技术检测PC mRNA和HBV DNA含量。结果：HepG2．2．15细胞的7种PCs均有不同程度的表达。加用重组TGFβ1后除PC5／6下调外，其余PCs均显著上调。尽管PC1／3和PC2上调最显著，但结合基础表达水平的分析显示furin和PACE4在TGFβ1，处理前后均为优势表达PCs。在HepG2．2．15细胞上，进一步使用PC抑制剂可消除TGFβ1抑制HBV复制的效应。结论：上调PCs mRNA表达是TGFβ1抑制HBV复制的重要中间环节。此结果对进一步探讨TGFβ1有效干预HBV复制具有积极意义。

英文摘要：

AIM ： To study the effect of proprotein eonvertases （PCs） on the transforming growth factor （TGF） β1 -induced inhibition of HBV replication. METHODS： HepG2. 2. 15 cells cultured regularly were exposed to recombinant TGFβ1 at concentration of 2 μg/L or 5 μg/L and/or PC inhibitor at concentration of 20 μmol/L for 18 h. The total RNA and HBV core particle DNA were extracted from these cells, and PC mRNA and core - associated HBV DNA were detected by real - time PCR technique. RESULTS ： The mRNA expression levels of 7 PCs in HepG2. 2. 15 cells were observed with various degrees. Recombinant TGFβ1 significantly up - regulated the mRNA expression of all PCs except for the down - regulation of PC5/6, though PC1/3 and PC2 were up - regulated most obviously. Furin and PACE4 were the predominant PCs before and after TGFβ1 exposure when the basic mRNA expression was taken into account. Further study showed that TGFβ1 - induced the inhibition of HBV replication was abrogated by PC inhibitor in HepG2. 2. 15 cells. CONCLUSION： TGFβ1 - induced the inhibition of HBV replication is mediated by the up - regulation of PCs, which might be of many implications in efficient interferences of TGFβ1 on HBV replication.