中文摘要：

目的筛选能提高硅酮橡胶弹性体（Sylgard184）与C2C12相容性的理想基质材料。方法Sylgard184双组分以10∶1的比例均匀混合,倒入6孔板的其中4孔,室温下静置固化,其余2孔做为空白对照培养组（A组）;固化后的Sylgard184表面依次经过以下处理：I型胶原包被（B组）、层黏连蛋白包被（C组）、多聚赖氨酸包被（D组）;未经包被（E组）,每组共6个样本。在不同基质材料修饰的Sylgard184表面培养C2C12细胞,利用倒置显微镜观察5组C2C12细胞的增殖、分化状态,流式细胞术（FCM）检测增殖培养48h后C2C12细胞的分裂增殖情况,RT-PCR检测增殖和分化培养48h后C2C12细胞内MyoD、myogenin mRNA的表达。结果Sylgard184材料存在细胞毒性,E组接种的C2C12细胞在24h内全部漂浮死亡;D组的大多数细胞出现死亡,仅少数贴壁存活;而B、C两组材料包被后明显减少Sylagard184的毒性,增强其表面与C2C12细胞的相容性,且C组细胞处于合成期的百分比以及增殖期的MyoD和分化期Myogenin基因mRNA的表达水平均显著高于A、B两组（P〈0.05）。结论经层黏连蛋白包被后的Sylgard184表面更有利于C2C12细胞的增殖及分化活性的表达。

英文摘要：

Objective An attempt was undertaken to acquire the ideal matrix material that can enhance the compatibility between silicone rubber elastomer（sylgard184） and murine C2C12 cells. Methods Two-component of Sylgard184 admixture to the ratio of 10∶1 was made then mixed into 4 wells of 6 Orifice plastic plate,solidification was carried out at room temperature,and the remaining 2 holes without coating were taken as blank control （group A）.The surfaces of 4 wells were coated by collagen type I,laminin and poly-lisine,respectively as group B,C and D,and the wells of uncoating as group E,every group had six samples. C2C12 cells were cultured on the surface of Sylgard184 coated with different matrix material,Cell proliferation,differentiation morphology of five groups of C2C12 cells were observed by the inverted microscopy. Flow cytometry （FCM） was used to detect the cell generation cycle of C2C12 cells after enrichment culture 48 hours.RT-PCR was used to test MyoD,myogenin mRNA expression,cell proliferation or differentiation cultured for 48 hours respectively that growed on coated or uncoated surfaces of Sylgard184.Results Sylgard184 showed the cytotoxicity,E group C2C12 cells were all floating and dead 24 hours after seeded.D group cells were only a few survived. While B and C groups can significantly reduce the toxicity of Sylagard184,and strengthen the compatibility between their surface and C2C12 cells. The ratio of S-phase cell in group C coated with laminin was significantly higher than that of in group A and B （P〈0.05）,as well as the mRNA expression of MyoD and Myogenin. Conclusion The data suggest that Sylgard184 modified by laminin is superior to that modified by other biomaterials,in terms of which can enhance the proliferation and differentiation of cultured C2C12 cells in vitro.