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The Stability of C-phycocyanin Doped Silica Biomaterials in UV Irradiation
  • ISSN号:1000-2413
  • 期刊名称:《武汉理工大学学报:材料科学英文版》
  • 时间:0
  • 分类:TN304.23[电子电信—物理电子学] TS202.3[轻工技术与工程—食品科学;轻工技术与工程—食品科学与工程]
  • 作者机构:[1]Department of Chemistry, School of Applied Science, University of Science and Technology, Beijing 100083, China
  • 相关基金:Funded by the National Natural Science Foundation of China (No. 20873005), Beijing Natural Science Fundation (No.2083028), and the Scientific Research Foundation tor the Returned Over.as Chinese Scholars, State Education Ministry and 422 Funds from University of Science and Technology Beijing
中文摘要:

综合 C-phycocyanins (CPC ) 做了硅石简历材料被 SEM 和赌注表面区域分析测量描绘。CPC 的形态学做了硅石简历材料显示硅石簇的表面被很多硅石粒子与一种平均尺寸形成在 30 和 40 nm 之间。硅石本身是有 2.95 nm 的平均毛孔直径的多孔的结构。有他们的直径的毛孔为 84.07% 的不到 5 nm 报道。另外, CPC 能作为一根荧光灯的蛋白质探针被利用监视蛋白质封装的影响并且在硅石矩阵学习矩阵和蛋白质相互作用和蛋白质的稳定性。蛋白质封装硅石材料的申请要求简历分子在潜在地相反的工业条件上保留简历活动和稳定性。在答案或在硅酸盐矩阵的 CPC 在相片漂白由紫外光线罐头结果照耀,而在硅石的蛋白质少些被影响。在缓冲区答案的 CPC 的测量相片损坏率常数是比在硅石矩阵的 CPC 的快 25 倍的。然而,在硅石矩阵或磷酸盐缓冲区的 CPC 的一生是未受影响的。这些研究建议 CPC 的那个陷阱进硅石矩阵能不仅维持他们的生物活动而且显著地改进他们的相片稳定性。

英文摘要:

The synthesized C-phycocyanins (C-PCs) doped silica biomaterials were characterized by the SEM and BET surface area analysis measurement. The morphology of C-PCs doped silica biomaterials indicates that the surface of the silica cluster is formed by a great number of silica particles with an average size of between 30 and 40 nm. Silica itself is a porous structure with the average pore diameter of 2.95 nm. Pores with their diameter less than 5 nm account for 84.07%. In addition, the C-PCs can be utilized as a fluorescent protein probe to monitor influence of the protein encapsulation and to study matrix and protein interaction and stability of protein in silica matrix. Application of protein encapsulation silica materials requires biomolecules to keep bioactivity and stability on potentially unfavorable industrial conditions. The C-PCs in solution or in silicate matrix irradiated by ultraviolet ray can result in photobleaching, whereas the protein in the silica is less affected. The measured photodamage rate constant of C-PCs in buffer solution is 25 times faster than that of C-PCs in silica matrix. However, the lifetime of C-PCs in silica matrix or phosphate buffer is unaffected. These studies suggest that entrapment of C-PCs into silica matrixes not only can maintain their biological activity but also noticeably improve their photostability.

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期刊信息
  • 《武汉理工大学学报:材料科学英文版》
  • 中国科技核心期刊
  • 主管单位:
  • 主办单位:武汉理工大学
  • 主编:
  • 地址:武汉市洪山区珞狮路122号
  • 邮编:430070
  • 邮箱:jwutms@mail.whut.edu.cn
  • 电话:027-87384113 87651870
  • 国际标准刊号:ISSN:1000-2413
  • 国内统一刊号:ISSN:42-1680/TB
  • 邮发代号:38-78
  • 获奖情况:
  • 国内外数据库收录:
  • 被引量:149