东篱科研大数据发现系统（DRDS）

位置：成果数据库 > 期刊 > 期刊详情页

染色体8q24和17q24区域与中国人前列腺癌的关联分析

ISSN号：1000-8578
期刊名称：肿瘤防治研究
时间：2012
页码：1442-1446
分类：R394[医药卫生—医学遗传学;医药卫生—基础医学] R737.25[医药卫生—肿瘤;医药卫生—临床医学]
作者机构：[1]北京大学第五临床医院(卫生部北京医院),北京100730, [2]卫生部北京医院泌尿外科
相关基金：国家自然科学基金资助项目（30972709,81061120527）; 北京医院重大基金资助项目（BJ-2010-30）; 卫生部部属医院临床学科重点资助项目（10120101）
相关项目：晚发阿尔茨海默病胆固醇调节基因遗传变异的识别和定义(中加健康研究合作计划)

关键词：染色体8q24区域, 17q24区域, 前列腺癌, 基因-基因互作, Chromosome 8q24 Chromosome 17q24 PCa , Gene-gene interaction

中文摘要：

目的探索染色体8q24区域（rs620861C、rs1447295A）和17q24区域（rs185996,G）风险等位基因与北方中国人前列腺癌的关联。了解其基因型与前列腺癌患者表型（年龄、肿瘤分期、PSA水平、Gl-eason评分、有无良性前列腺肥大）的关联,分析基因之间的交互作用。方法采用病例-对照设计,收集前列腺癌患者临床表型信息;采用PCR-HRM和测序技术对各位点进行了分型,并对基因型在病例组和正常对照组中的分布进行了年龄匹配的病例-对照间比较（病例组：124人,对照组：138人）及基因交互作用分析;对病例组中基因型在不同临床表型中的分布进行了比较。结果在病例-对照之间三个风险位点的基因型和等位基因频率分布差异未见有统计学意义;在病例组中各位点与前列腺癌表型之间未见有显著性关联;未能检测到三个风险位点之间有交互作用。结论染色体8q24区域（rs620861C、rs1447295A）和17q24区域（rs185996G）可能与北方中国人患前列腺癌的遗传风险无关联。

英文摘要：

Objective To explore whether the risk alleles in Chromosome 8q24（rs620861C, rs1447295A） and Chromosome 17q24（rs1859962G）are associated with prostate cancer in Chinese population to investigate the association between genotypes and phenotypes （age, T staging of neoplasm, PSA level, Gleason scores and suffering from BPH or not）of PCa patients, to analyse the the interactions among the genes. Methods A case-control study was performed to collect information of PCa patients＇clinical features. By using polymerase chain reaction-high resolution melting curve（PCR HRM）combined with DNA sequencing,each locus was genotyped and examined,and the distribution of each locus＇ genotype was compared in an age-matched case-control method（124 cases, 138 controls）：and the gene-gene interaction analysis was done. The contribution of patients＇ genotypes in different clinical features was done. Results None of the three variants was observed to show significant difference in the distribution of genotype and gene frequency between cases and controls,neither did they show significant association with PCa phenotypes in cases. There was no significant gene-gene interactions being detected, either. Conclusion Chromosome 8q24（rs620861C,rs1447295A）and Chromosome 17q24（rs1859962G）may have no association with pros- tate cancer in Chinese population.

同期刊论文项目