中文摘要：

以双子叶模式植物拟南芥（Arabidopsis thaliana）突变体crylcry2为实验材料，用舍有激活标记质粒DSK1015的农杆菌浸花进行转化，构建了拟南芥T-DNA插入突变体库．通过筛选和观察分析，获得了一些开花时间比crylcry2明显延迟或明显提早的突变体．采用IPCR（inverse PCR）和TAIL-PCR（thermal asymmetric interlaced PCR）等方法，鉴定了这些突变体T-DNA插入位点的基因组旁邻序列，并采用半定量RT-PCR对插入位点两侧基因的mRNA水平进行了分析，初步鉴定了与开花相关的候选基因，为进一步研究其功能，深入研究隐花素调节光周期开花的作用机制奠定了基础．

英文摘要：

The Arabidopsis mutant crylcry2 was used as experimental material in the study. A library of Arabidopsis mutants was constructed by floral dip method, Agrobacterium tumefaciens with an activation tagging vector pSKI015 and herbicide Basta as a selection marker was transformed into the mutant crylcry2. By screening and observation, some mutants with visible morphological phenotypic variations were obtained. IPCR（inverse PCR） and TAIL-PCR（thermal asymmetric interlaced PCR） were used to identify the flanking genomic sequences of mutated target genes. At the same time, the semi-quantitative RT-PCR was used to analyze expression of the mutant genes as the first step towards the function of mutant genes. The result showed in the paper laid the foundation for further study of photoperiod regulation by cyptochrome.