中文摘要：

目的：研究复方肝舒康各提取部位的体外抗乙肝病毒（HBV）作用。方法：取各萃取部位作用于乙肝病毒DNA转染人肝癌细胞系2215细胞,以细胞病变（CPE）考察药物对该细胞的毒性,采用酶联法（ELISA）检测药物对2215细胞分泌的表面抗原（HBsAg）和e抗原（HBeAg）表达的抑制作用。结果：石油醚部位、乙酸乙酯部位和正丁醇部位的半数毒性浓度（TC50）分别为125、375、62.5μg/mL。最大无毒浓度（TC0）分别为62.5、125、31.3μg/mL;石油醚部位、乙酸乙酯部位对2215细胞的HBsAg表达抑制作用的半数有效浓度（IC50）分别为48.6、14.0μg/mL,对2215细胞的HBeAg表达抑制作用的半数有效浓度（IC50）52.4、19.7μg/mL,而正丁醇部位均大于125μg/mL。石油醚部位的治疗指数（TI）值分别为2.57和2.43,乙酸乙酯部位的治疗指数分别为26.7和19.04,正丁醇部位均小于2。结论：复方肝舒康提取物乙酸乙酯部位体外抗乙肝病毒的作用最强,石油醚部位抗乙肝病毒作用较弱,正丁醇部位对乙肝病毒无作用。

英文摘要：

Objective：To study the anti-HBV effect in vitro of each extract from compound Gan Shu Kang.Methods：The toxicity was investigated with cytopathic effect（CPE） by enzyme linked immuno-adsorbed assay（ELISA）,the inhibitory effect of each extract on HBsAg and HBeAg secreted by 2215 cell line infected by HBV DNA was evaluated.Results：The half of the toxic concentration（TC50） of petroleum ether,ethyl acetate and n-butanol was 125,375 and 62.5 μg/mL,respectively.The maximum nontoxic concentration（TC0） was 62.5,125 and 31.3 μg/mL,respectively.The medium effective concentration（IC50） of petroleum ether and ethyl acetate on 2215 cellular HBsAg express the inhibitory effect was 48.6 and 14.0 μg/mL,HBeAg was 52.4 and 19.7 μg/mL,while the IC50 of n-butanol for HBsAg and HBeAg was more than 125 μg/mL.The therapeutic index（TI） of petroleum ether was 2.57 and 2.43,ethyl acetate was 26.7 and 19.04,n-butanol was both less than 2.Conclusion：The anti-HBV effect of ethyl acetate extract of compound Gan Shu Kang is better than that of petroleum ether,while the n-butanol extract shows no effect.