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中文摘要：
在顺序注射系统中引入C18固相萃取柱进行生米卡链霉菌菌落样品的纯化富集，建立了快速测定菌落中麦白霉素的分光光度法。用超声波破碎菌落细胞后，将样品溶液通过固相萃取柱，用少量甲醇洗脱吸附在C18微珠上的麦白霉素，232am检测。选样量为2ml，洗脱液体积为100μL，麦白霉素的富集倍率为14；线性范围为0．1～10μg／mL，检出限为0．02μg／mL，RSD为2．5％（0．5μg／mL，n=7）。菌落样品中麦白霉素的加标回收率为96％～100％。
英文摘要：
A rapid spectrophotometric method for the determination of meleumycin in colonies of streptomyces mycarofaciens was developed via solid-phase extraction in a sequential injection system. After meleumycin was released from the cell by ultrasonic lysis, 2.0 mL of sample solution was directed to flow through a C18 packed mini-column. The meleumycin retained onto the column was afterwards eluted with 100μL of methanol and the eluate was monitored at a wavelength of 232 nm. With a 2 mL sampling volume, an enrichment factor of 14 was achieved within 0.1 - 10 μg/mL, and a detection limit of 0.02 μg/mL was derived along with a RSD of 2.5 % at 0.50μg/L. Spiking recoveries of 96% - 100% for meleumycin in three colony samples were obtained.
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