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中文摘要：
目的应用计算机在线预测软件分析细粒棘球绦虫AgB5抗原蛋白的二级结构,预测其可能形成的B细胞表位和T细胞表位,为研制安全、高效的新型包虫病基因疫苗奠定基础,也为包虫病的免疫学治疗提供新的理论依据。方法采用计算机在线预测软件SOPMA对细粒棘球绦虫AgB5抗原蛋白的二级结构进行预测和分析;采用LEPS和ABC Pred网络软件结合其表面特性、亲水性、柔韧性、可及性、可塑性等对其B细胞表位进行预测和分析;采用IEBD和SYFPEITHI在线软件对细粒棘球绦虫AgB5抗原蛋白的MHC-I类HLA-A 0201限制性T细胞表位进行预测和分析。结果采用计算机在线预测软件预测细粒棘球绦虫AgB5抗原蛋白的二级结构中α螺旋结构占76.47%,β-折叠占5.88%,无规则卷曲占17.65%。结合其表面特性、亲水性、柔韧性、可及性以及可塑性等分析得出分值较高的3个B细胞表位区域,分别为：6~14、28~33和48~52氨基酸序列,较易形成B细胞表位;通过MHC-I类HLA-A 0201限制性T细胞表位分析得出分值较高的4个T细胞表位区域,分别为：9~13、24~29、38~45和51~59氨基酸序列,较易形成T细胞表位。结论通过生物信息网络技术方法分析确定细粒棘球绦虫AgB5抗原蛋白分别存在3个B细胞抗原表位和4个T细胞抗原表位,可为细粒棘球绦虫AgB抗原性研究和高效表位疫苗研发提供参考,为临床包虫病的免疫诊断、药物治疗提供新的靶标。
英文摘要：
Objectives To use computer-based and online software to predict and analyze the T cell and B cell epitopes and secondary structure of Echinococcus granulosus antigen B5（AgB5）in order to determine and identify dominant epitopes and to lay the foundation for development of new,safe,and effective multiple antigen peptide vaccines. Methods The secondary structure of AgB5 protein was determined using the software SOPMA,B cell epitopes were predicted using the software LEPS and ABCPred,and T cell epitopes were predicted using the software IEBD and SYFPEITHI.Results Results indicated that anα-helix accounted for 76.47% of the secondary structure of AgB5 protein,aβ-sheet accounted for 5.88%,and a random coil accounted for 17.65%.Three potential B cell epitopes were readily identified as sequences of amino acids 6-14,28-33,and 48-52.Four potential T cell epitopes were readily identified as sequences of amino acids 9-13,24-29,38-45,and 51-59. Conclusion Bioinformatic methods were used to verify the existence of 4different T cell epitopes and 3Bcell epitopes on AgB5.These findings can serve as a reference for further study of AgB5 and development of effective epitope-based vaccines.
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