中文摘要：

为建立甜瓜（CucumismeloL．）简便易行转化频率高的转基因技术，以甜瓜品种河套蜜瓜为受体材料，以含gus基因的植物双元表达载体pPZP221为外源基因供体，进行了花粉管通道法转基因研究．自交授粉后分别于1、2、3、4、5、6、7、8、9、10h，切去柱头上端1／3部分，立即滴加质粒DNA溶液，果实成熟后收获转化种子．对T0代植株进行PCR检测，结果表明不同时间处理所得到的T0代植株均具有较高的转化频率，其中授粉后7h滴加DNA溶液所获得的转化率为28．3％．对一部分PCR阳性的T0代植株基因组DNA进行Southern杂交分析，结果表明所有样品均出现特异性杂交条带，证明外源基因已整合到受体植物基因组中．

英文摘要：

In order to establish a simple, practical technique with high transformation frequency in melon （Cucurnis melo L. ）, the pollen-tube pathway method was used for melon transformation. The melon cultivar Hetao was used as a recipient and the plant binary vector pPZP221 containing gus gene was used as donor DNA. The plasmid DNA solution was applied to the cut surfaces of stigmas at 1,2,3,4,5,6,7,8,9,and 10 h after hand-pollination,respectively, The PCR results show that the transformation rate of all T0seedlings is very high and the highest rate is up to 28.3% at 7 h after pollination. Southern blot analysis confirmed that foreign DNA was indeed inserted into the genome of transgenic T0seedlings.