为研究MBR(膜生物反应器)降解SA(对氨基苯磺酸)的性能,构建一套连续流MBR,针对ρ(SA)为25 mg/L的模拟废水进行处理,并通过高通量测序对MBR运行过程中微生物群落特征变化进行生物学层面的分析.结果表明:经过31 d的启动驯化后,SA基本可以完全降解,COD(Cr)、NH4+-N、TN和TP的去除率分别为87.63%±5.95%、91.94%±8.80%、32.38%±11.6%和85.69%±13.82%.对驯化及稳定运行阶段的污泥进行微生物菌群分析结果表明,在"门"水平上主要的微生物菌群为拟杆菌门、变形菌门和绿弯菌门,其中拟杆菌门是处理含SA废水的优势菌群.在"科"水平上,噬几丁质菌科、腐螺旋菌科、红环菌科、丛毛单胞菌科和拜叶林克氏菌科为主要的微生物菌群,随着反应器的长期驯化和运行,噬几丁质菌科逐渐成为反应器中优势菌群.研究显示,MBR对SA、CODCr、NH4+-N和TP都有很好的去除效果,拟杆菌门和噬几丁质菌科分别为处理SA的优势"门"和"科".
A continuous-flow membrane bioreactor (MBR) was operated for the treatment of simulated wastewater containing 25 mg/L sulfanilic acid (SA) to study SA biodegradation. After 31 d acclimation period, SA was degraded completely, and the removal efficiencies of C0DCr, NH4 + -N, TN and TP were 87. 63% ± 5. 95% ,91. 94% ± 8. 8 0 % ,32. 38% ± 11. 6 % ,and 85. 69% ± 13. 8 2 %, respectively. At the same t ime, the microbial communities in the sludge of different phases were investigated. At the phylum level, Bacteroidetes, Proteobacteria and Chlorobia were found to be the main taxa in the reactor, and Bacteroidetes were the dominant phylum responsible for the SA degradation. At the family level, it was found that the microbial communities were dominated by Chitinophagaceae, Saprospiraceae, Rhodocyclaceae, Comamonadaceae and Beijerinckiaceae, and Chitinophagaceae became more dominant during long-term operation. The results showed that the good removal efficiency of SA, C0DCr, NH4 +-N and TP was achieved, and Bacteroidetes and Chitinophagaceae were the dominant phyla and families for the SA degradation, respectively.