中文摘要：

目的：在体外探讨前列腺环素E2对神经干细胞增殖分化的影响及可能的分子机制。方法：原代培养小鼠胚胎神经干细胞。分为溶剂对照组；100nmol／L、1μmol／L前列腺环素E2处理组；1μmol／L前列腺环素E2加15μmol／LXAV939处理组。上述各组细胞在无血清增殖培养基中培养两天后，行BrdU、PY489染色，观察神经干细胞的增殖及Wnt信号的激活；在含2％胎牛血清的分化培养基中培养四天后，行Tuj／GFAP，CNPase染色，观察神经干细胞的分化。结果：100nmol／L，1μmol／L前列腺环素E2可分别使神经干细胞的增殖增加约86％和195％，及其向神经元方向的分化增加约85％和257％，抑制向星形胶质细胞方向的分化，但不影响向少突胶质细胞方向的分化。100nmol／L，1μmol／L前列腺环素E2可使核型p—catenin阳性细胞的百分率分别增加约31％和91％。15μmol／LXAV939可显著降低1μmol／L前列腺环素E2诱导的神经干细胞增殖及其向神经元方向的分化。结论：前列腺环素E2可在体外通过Wnt／β-catenin信号促进神经干细胞增殖，并诱导其向神经元方向分化。

英文摘要：

Objective： To assess the effects of prostaglandin E2 （PGE2） on the proliferation and differentiation of neu- ral stem cells, and the corresponding mechanisms in vitro. Methods： Primary neural stem cells were treated with （ 1 ） ve- hicle control; （2） 100 nmol/L, 1 μmol/L PGE2; （3） 1 μmol/L PGE2 plus 15 μmol/L XAV939. After 2d＇s culture in proliferative medi μmol/L, immunostaining of BrdU and PY489 were performed to assess cell proliferation and Wnt signa- ling activation. After 4d＇s culture in the differentiating medium, immunostaining of GFAP, Tujl and CNPase were con- ducted to evaluate the differentiation of neural stem cells. Results： 100 nmol/L and 1 μmol/L PGE2 significantly pro- mote the proliferation of neural stem cells by about 86% and 195%, and neuronal differentiation by 85% and 257%, suppress the astrocytic differentiation of neural stem cells, but not the generation of oligodendrocyte. 100 nmol/L and 1 μmol/L PGE2 significantly increase the percentages of nuclear β-catenin by 31% and 91%. Wnt signaling inhibitor, XAV939 can significantly decrease the PGE2-induced proliferation and neuronal differentiation of neural stem cells. Con- clusion： PGE2 can stimulate neurogenesis through Wnt/β-catenin signaling in vitro.