中文摘要：

目的：研究双夏汤中不同配比的半夏配伍夏枯草对葫芦巴碱、尿嘧啶提取率的影响,探索其变化规律,指导双夏汤的合理配伍。方法：采用Welch Ultimate AQ-C18色谱柱（4.6 mm×250 mm,5μm）,以乙腈-水为流动相,梯度洗脱,流速为1.0 m L·min^-1,检测波长为265 nm,柱温25℃,利用建立的HPLC法测定葫芦巴碱、尿嘧啶的提取率,比较得出不同配比对2种成分提取率的影响。结果：葫芦巴碱进样量在0.872—4.36 ng,尿嘧啶在10.8—50.4 ng之间线性关系良好,平均加样回收率分别为96.53%—101.48%,RSD均在0.18%—4.26%。双夏汤配伍后较半夏原药葫芦巴碱提取率降低,尿嘧啶提取率增加。结论：复方双夏汤不同配伍对葫芦巴碱和尿嘧啶的提取率有一定影响,最佳配比值得深入研究。

英文摘要：

This article was aimed to study the effect of pinellia combined with prunella vulgarisin of different ratios on the extraction yield of trigonellin and uracil, in order to investigate the changing disciplines and reasonable combination of Shuangxia Soup. A Welch Ultimate AQ-C18 column（4.6 mm×250 mm, 5 μm） was used in the study. The mobile phase, consisting of solvent A（water） and solvent B（acetonitrile） was programmed as a linear gradient. The flow rate was 1.0 m L·min^-1. And the detection wavelength was 265 nm. The column temperature was 25℃. HPLC was used in the determination of extraction yield of trigonellin and uracil for the comparison among different compatibilities. The results showed that the linearity was achieved in the range of 0.872—4.36 ng for trigonelline, and 10.8—50.4 ng for uracil. The average recoveries were 96.53%—101.48%, with RSD of 0.18%—4.26%. Compared with pinellia, the extraction yield of trigonelline was reduced and the extraction yield of uracil was increased after the compatibility. It was concluded that different compatibilities of Shuangxia Soup had effects on the extraction yield of trigonelline and uracil. The best compatibility ratio still requires in-depth study.