中文摘要：

多梳蛋白（polycomb group,PcG）是一类对生物有机体生长发育很重要的转录抑制因子,参与有机体的发育调控。Ring和YY1结合蛋白（Ring and YY1 binding protein,RYBP）是在小鼠（Mus musculus）和果蝇（Drosophila melanogaster）中推断的PcG蛋白成员。基于生物信息学分析方法,设计引物克隆了家蚕的RYBP基因（BmRYBP）;序列结构分析显示该基因有一个420 bp的完整ORF,由3个外显子和2个内含子组成,编码139个氨基酸,预测其蛋白质分子质量为15.4 kD,等电点为10.36,N端的20～44 aa有一个与蛋白间相互作用有关的锌指结构域（ZnF-RBZ）;半定量RT-PCR分析显示,该基因在家蚕5龄3 d幼虫生殖腺中高水平转录;基于GAL4/UAS双元系统,构建了具有BmRYBP序列反向重复结构的转基因干涉表达载体,通过显微注射家蚕早期胚胎,获得了UAS系统的转基因家蚕后代,为研究BmRYBP在家蚕生长发育过程中行使的功能奠定了基础。

英文摘要：

The polycomb group（PcG） proteins are transcriptional repressors essential for the normal growth and development of multicellular organisms,being involved in the regulation of organismal development.Ring and YY1 binding protein（RYBP） was considered as a member of PcG protein family in Mus musculus and Drosophila melanogaster.Based on bioinformatics analysis,primers were designed to clone the silkworm（Bombyx mori） RYBP（BmRYBP） gene.Sequence analysis showed that this gene has 3 exons and 2 introns.Its intact ORF has 420 bp which encodes 139 amino acid residues with predicted molecular weight of 15.4 kD and pI of 10.36.The protein＇s N-terminal No.20 to 44 amino acids form a zinc-finger domain（ZnF-RBZ） which is considered to be involved in protein-protein interaction.Semi-quantitative RT-PCR analysis showed high level of transcription of this gene in gonad of day 3 silkworm larvae of the 5th instar.Based on GAL4/UAS binary system,a transgenic interference vector containing inverted repeat structure of BmRYBP sequence was constructed and micro-injected into early embryos of silkworm.As a result,transgenic silkworm progeny harboring the UAS system was obtained,establishing a good basis for functional characterization of BmRYBP in silkworm growth and development.