中文摘要：

背景：人工支撑脂双层膜是最接近细胞膜的体外仿生模型,构建结构与功能均类似于真实细胞膜的脂双层膜具有十分重要的意义.目的：探索表面均匀且流动性高的支撑脂双层膜的最佳制备条件并检测指标.方法：采用薄膜挤压法,分别以孔径为0.1,0.2μm的滤膜对脂质体分子过滤10次,保留未过滤样品作为对照组,通过原子力显微镜对其表面粗糙程度进行检测,探讨制备均匀程度较高的脂双层膜所需滤膜的最佳孔径.将160μL卵磷脂（10 g/L）分别与5,10,25μL荧光磷脂（1 g/L）进行混合,通过荧光漂白恢复技术对脂双层膜的流动性进行检测,探讨卵磷脂与荧光磷脂的最适比例.结果与结论：原子力显微镜实验结果显示,经过孔径为0.1μm滤膜过滤所得的脂双层膜表面均匀程度最高（P〈0.01）,其表面均方根粗糙度为（0.432±0.181）nm,当滤膜孔径大于0.1μm时,脂双层膜表面均匀程度差且存在较多的未铺展开脂质体囊泡.荧光漂白恢复实验结果显示,采用孔径为0.1μm滤膜过滤的脂双层膜荧光恢复程度最高,当卵磷脂（10 g/L）与荧光磷脂（1 g/L）体积比为160：10时,采用孔径为0.1μm滤膜过滤的脂双层膜荧光恢复程度达到90%,且扩散系数大于1μm2/s,符合优质脂双层膜的标准.实验提示利用薄膜挤压法制备脂双层膜时,卵磷脂与荧光磷脂的最适比例以及最佳滤膜孔径.

英文摘要：

BACKGROUND： The artificial supported lipid bilayer membrane is the most similar in vitro biomimetic model of the cell membrane. The artificial high-quality lipid bilayer membrane will provide a critical tool for the study of the microstructure of biological membranes, cell signal transduction, biofilm sensors and drug carriers.OBJECTIVE： To explore the optimal preparation conditions of the lipid bilayer membrane with uniform surface and high mobility and its detection indexes.METHODS： By thin film extrusion, liposomes were filtrated 10 times through the filter membrane with the pore diameter of 0.1 and 0.2 μm respectively, with the unfiltered sample as control. The root mean squared roughness of lipid bilayer membrane was detected by atomic force microscopy to explore the optimal pore diameter of the filter membrane for preparation of the lipid bilayer membrane with high uniformity. 160 μL egg-PC （10 g/L） and 5, 10 or 25 μL NBD-PC were mixed separately. The mobility of lipid bilayer membrane was detected by fluorescence recovery after photobleaching （FRAP） to explore the optimal proportion of egg-PC and NBD-PC.RESULTS AND CONCLUSION： The results of atomic force microscopy showed that the lipid bilayer membrane through the 0.1 μm filter membrane had the highest degree of uniformity （P 〈0.01）, with the root mean squared roughness of（0.432±0.181） nm. By using the filter membrane whose pore diameter was greater than 0.1 μm, the surface uniformity of the lipid bilayer membrane was poor and there were more liposome vesicles on the surface. The FRAP results showed that the lipid bilayer membrane through the 0.1 μm filter membrane had a higher degree of fluorescence recovery. When 160 μL egg-PC （10 g/L） and 10 μL NBD-PC were mixed and filtrated through the 0.1 μm filter membrane, the degree of fluorescence recovery of the lipid bilayer membrane reached 90% and the diffusion coefficient was greater than 1 μm2/s which met the standard of high-quality lipid bilayer membrane. These re