以葡萄中的蔗糖转运蛋白为主要研究对象,结合了功能研究较为深入的拟南芥和水稻蔗糖转运蛋白家族序列,分析讨论了这些基因在启动子区域顺式作用元件的异同,以及这些差异可能对mRNA的转录带来的影响;同时,根据蔗糖转运蛋白氨基酸序列对其家族进行了分类,分析了不同亚类蔗糖转运蛋白基因结构的特点;最后还对蔗糖转运蛋白家族中氨基酸的保守性进行了分析。这些分析将为后续蔗糖转运蛋白功能基因组学的研究以及通过基因工程技术精确调节植物代谢提供一定的依据。
This study mainly focused on grape sucrose transporters (SUCs).Depending on the studies of SUCs of arabidopsis and rice,we analyzed the cis-acting element in SUCs’ promoter sequences,predicted their affections on mRNA transcription.We classified SUCs to three subtribes according to their amino acid sequences,and described gene structure of every subtribe.Amino acid conservation in different SUCs was calculated subsequently.This study throws light on the further functional genomics researches of SUCs and accurate control of plant metabolism by gene technology.