目的:探讨利用内生真菌促进菊花生产的措施。方法:以内生真菌球毛壳菌(编号C4)、葡萄孢真菌(编号C1)共生培养菊花,检测其产量和品质。结果:与对照相比,C4菌处理组鲜菊花和干菊花产量分别增加24.81%、7.59%,C1菌处理组鲜和干产量分别增加17.08%、6.87%。C4、C1菌处理组菊花中总黄酮含量比对照组分别增加31.79%、8.55%,且C4菌处理组与对照组相比达到显著水平。菊花中粗挥发油含量比对照组分别增加13.21%、18.19%。经气相色谱检测发现内生真菌C4、C1菌处理组样品中占总挥发油含量大于1%各组分含量总和分别比对照增加10.42%、8.90%,且内生真菌处理组与对照组之间各组分含量存在差异。结论:内生真菌与菊花建立共生关系后,可以提高菊花产量和品质。
Objective:To enhance the flowers yield of Chrysanthemum morifolium by endophytic fungi.Methods:Endophytic fungi(Chaetomium globosum strain C4 and Botrytis sp.strain C1)were inoculated to the plantlets which were planted in the pots.The output of the flowers was measured,the total flavonoids and essential oil contents of the flowers were determined.Results:Compared to that of the control,fresh and dry outputs of the fungi C4 group increased 24.81%,7.59%,fresh and dry outputs of the fungi C1 group increased 17.08%,6.87%.Total flavonoids content of the fungi C4 group was higher than that of the control remarkably,fungi C4,C1 groups flowers total flavonoids content increased 31.79%,8.55% compared to that of the control.Essential oil content of the fungi C4,C1 groups increased 13.21%,18.19% respectively.The content percentage of various essential oil components of the fungi C4,C1 groups increased 10.42%,8.90% compared to that of the control respectively.There were differences among the content percentage of various essential oil components of fungi C4,C1 treated group and the control′s.Conclusion:The two fungi could build the symbiosis relation with the Chrysnthemum morifolium,which may cause them to enhance the output and quality finally.