中文摘要：

为了克隆小麦干旱应答基因，构建了干旱诱导的小麦cDNA文库，并从文库中分离了一个DREB类转录因子基因TaDREB6。序列分析表明，TaDREB6具有一个837 bp的开放阅读框和242 bp的3’非编码区，推测的氨基酸序列中含有一个高度保守的AP2／EREBP结构域。采用该基因特异引物PCR技术对一套中国春缺体-四体材料进行扩增，将TaDREB6定位于3A染色体上，这是首次将一个小麦DREB基因定位在特定的染色体上。RT-PCR分析表明，TaDREB6基因受干旱胁迫诱导表达；亚细胞定位结果表明，TaDREB6-hGFP融合蛋白定位于细胞核中。上述结果说明，小麦TaDREB6基因编码的蛋白可能在细胞核内对干旱胁迫应答反应起调控作用。

英文摘要：

In search for genes response to drought stress in wheat （Triticum aestivum L. ）, a cDNA library was constructed from drought-treated wheat seedlings, and a DREB-like transcription factor gene, TaDREB6 was isolated. TaDREB6 had an open reading frame of 837 bp with a 3＇-UTR of 242 bp, with a highly conserved AP2/EREBP domain in the encoded putative protein. Using a nullisomic-tetrasomic series of T. aestivum L. cv. Chinese Spring, the TaDREB6 gene was located on chromo-some 3A. The result of RT-RCR indicated that TaDREB6 gene was obviously induced by drought stress, suggesting an important role of TaDREB6 in response to drought stress in wheat. Subcellular localization assay indicated the TaDREB6-hGFP fusion protein was targeted in the nucleus. Above results suggested that TaDREB6 transcription factor might be involved in drought stress response and functioned in the nucleus.