中文摘要：

目的：探讨2型重组腺相关病毒（AAV2）载体能否有效转导人骨髓CD34^＋细胞与间充质干细胞。方法：构建、包装、纯化携带绿色荧光蛋白基因的2型重组腺相关病毒（rAAV22／GFP），转染骨髓CD34^＋造血干／祖细胞和间充质干细胞，转导后48h在荧光显微镜下观察GFP的表达，并比较羟基脲（HU）处理前后rAAV2／GFP对CD34^＋细胞的转染效率。结果：rAAV2／GFP对CD34^＋细胞的转染效率为5．3％±1．7％。经羟基脲预处理后达13．2％±2．8％，rAAV2／GFP对间充质干细胞的转染效率为23％±3．6％。结论：rAAV2对间充质干细胞的转染效率高于骨髓CD34^＋细胞，羟基脲预处理可以明显提高rAAV2对CD34^＋细胞的转染效率。

英文摘要：

Objective To investigate the transduction efficiency of recombinant adeno-associated virus 2 （rAAV2） in human bone marrow CD34^＋ hematopoietic stem/progenitor cells and mesenchyme stem cells. Methods The rAAV2 containing green fluorescent protein genes （rAAV2/GFP） were constructed, packaged and purified. CD34^＋ hematopoietic stem/progenitor cells and mesenchyme stem cells were infected with the rAAV2/GFP. After transduction for 48 hours, the expression of GFP was detected under fluorescence microscope. Furthermore, the transduction efficiency of AAV transduced CD34^＋ with hydroxyurea （HU） pretreatment and that of untreated were compared. Results GFP genes were expressed in 5.3% ± 1.7% CD34^＋cells. After pretreatment with HU, the expression of the GFP gene in CD34^＋ cells increased to 13.2% ±2.8% , and 23%±3.6% mesenchyme stem cells expressed the GFP gene. Conclusion The transduction efficiency of mesenchyme stem cells is higher than that of CD34^＋ hematopoietic stem/progenitor cells. HU pretreatment can obviously increase the transduction efficiency of CD34^＋ hematopoietic stem/progenitor cells.